Re: Microfluidics Chat Thread WAS: [DIYbio] Paper request

On Sat, Dec 27, 2014 at 7:45 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> I recently made this in implicitCAD, I haven't made anything else
> yet... but I will give your fluidic a shot!
> https://github.com/nmz787/microfluidic-cad/blob/master/implicitCAD/output/sinusoidal_mixer.png

I pooled the papers together that the fabrication steps refer to when
talking about the symmetrical birfurcation for dispersion (which the
references don't mention, at least using those keywords):
diyhpl.us/~nmz787/pdf/Microfabricated_arrays_for_high-throughput_screening_of_cellular_response.pdf
http://diyhpl.us/~nmz787/pdf/Versatile_fully_automated_microfluidic_cell_culture_system.pdf
http://diyhpl.us/~nmz787/pdf/Dynamic_single_cell_culture_array.pdf

And the original:
http://diyhpl.us/~nmz787/pdf/Culture_and_chemical-induced_fusion_of_tobacco_mesophyll_protoplasts_in_a_microfluidic_device.pdf


My progress since yesterday:

https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.jpg

(for some reason it doesn't render for me unless I download it then
open it in my browser, or inkscape)
https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device.svg

https://raw.githubusercontent.com/nmz787/microfluidic-cad/master/implicitCAD/output/tobacco_mesophyll_protoplast_fusion_device__3D.png

and the source code:
https://github.com/nmz787/microfluidic-cad/blob/master/implicitCAD/tobacco_mesophyll_protoplast_fusion_device.escad

I am new to using this CAD software, and I am not sure it is something
I want to continue using or not... as you can see the JPG (which is a
snapshot of an SVG file the CAD tool generated) is not perfect around
the posts (not to mention I didn't finish the end of the channel
exactly as the paper did). I think I can turn up the $quality
variable, which makes rendering take a bit longer but might fix the
weird looking posts completely. I think the CAD tool can emit gcode,
which the UV laser engraver can take as input... so I guess I have to
investigate a g-code viewer to make sure it looks OK before exposing
some resist.

I think the CAD code should be easily ported to other CAD tools like
openSCAD or freecad, but I haven't made any progress with them as
easily as I have with this implicitCAD so far. More to learn and play
with!

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