[DIYbio] Re: DIY Turbidostat

Hi everyone,

thanks to JT first for his amazing project and sharing it as well as the content around it. Just to compelete this thread here a few links of more turbidostat and chemostat systems that were built by the Dunham and the Klavin lab:

DIY Turbidostat Array:
https://depts.washington.edu/soslab/turbidostat/pmwiki/pmwiki.php
DIY Chemostat Array:
http://dunham.gs.washington.edu/DunhamLabMinistats.pdf

I find these projects really inspiring especially as considering this patent solves the problems of biofilms/ dilution resistant strains! https://www.google.de/patents/WO2003004656A9?dq=mutzel&ei=im-mVI2uK4e-PK6OgMgO&cl=en
So by using two culture vessels and transfering the culture from one to the other vessel and cleaning out the one that is not in use - you can actually grow ou bugs for unlimited generations. So basically you can adapt bugs to grow on any waste media by just waiting until they are adapted (talking it easy).

I am currently planning to built a low cost model of the Evolinator and optionally combing it with another vessel. We will see :). However, I intend to culture anaerob archeae that grow really slow (doubling time 4h) so I will start with the normal Evolvinator set up under a DIY anaerobic chamber. Does anybody has experience with anaerobes? Are they suitable for such an automated culturing endeavor?

Cheers and thanks for feedback

Johann

Am Freitag, 28. Oktober 2011 04:19:41 UTC+2 schrieb Jt:

Hello,

New to the DIYBio group.

I have created a turbidostat that I would like to share with the
community. In brief, it uses an Arduino to control a peristaltic pump
based on OD readings, includes temperature control as well as an
aquarium pump for aeration. Additionally, I have a version that
forgoes the pump and relies on air pressure and valves to move fluid
through the system. The system currently uses the Arduino Ethernet
Shield and HTML interface to control it, though an interface over
serial could be easily written for anyone who wants to go into the
code.

Besides the Arduino code, I have CAD drawings for the housings,
EAGLE .brd and .sch files for the additional "Turbidostat Shield" I
made that contains the electrical components, as well as the parts
list. I haven't created the How-To yet because I'm not sure where the
best place to publish the designs are and what the demand is from the
community. I read that there was an open source turbidostat project on
DIYbio, but cannot find its current progress.

In any case, the device is relatively cheap (especially depending on
what you have on hand and where you want to cut corners), and I think
it would be helpful the community.

Any direction would be greatly appreciated.

jt