[DIYbio] Re: saccharomyces cerevisiae potentialities

Awesome- that sounds like a dream job!

Whereabouts are you located? We've been looking forward to doing some brew hacking.

One fun thing to follow up on would be the symbiosis between aromatic beer yeasts and fruit flies. Use some of your favorite beer to attract fruit flies, capture some of the wild yeasts the flies carry, sequence their ATF1 gene, and make a library of ATF1 mutant yeasts with different aromatic properties.

You could do the same with a bunch of other known aromatic compound producing enzymes. Or you could use subtractive techniques to sequence the parts of the genome that differ from standard S. cerevisiae.

Patrik

On Friday, December 26, 2014 9:29:46 AM UTC-8, Nick M. wrote:

All, 

Hope everyone had lovely and peaceful holidays. 

I've been lurking the group for ~16 months now. So far I've done a GFP plant, selective breeding on plants, selective breeding on brewer's yeast, and a few other random projects. I want to thank you all for the invaluable depth of knowledge that is available here.

I am a brewer by profession and have recently taken over a brewery with considerable corporate backing, corporate has told me 'Do what you want, don't break anything, and most importantly make great beer.' 

I take this to me I can build my own synbio lab, I am confident that is within my AOR. 

 I was hoping to receive some input from you all on interesting directions to go with S. cerevisiae. 

I have seen the iGEM projects on beer, caffeine, limonene(particularly interesting to me), and kumamolisin, and all of those are things I would like to work towards. 

Any ideas on what I can do in the interim whilst trial running my lab and knowledge base? Looking for projects that would be valuable to me in either an academic or practical regard that would build a good framework of experience to operate on in a professional standard. I also feel indebted to the community and must offer my position to the benefit to the diybio community once it becomes a position of value. 

Cheers,
Nick 

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