Re: [DIYbio] Re: Is it possible for a Homeworker to create a thermosensitive yoghurt?

As the original question was:
Q: "Is it possible for a Homeworker to create a thermosensitive yoghurt?"

My answer is: "No."
Oh, even if it's "just" for a school pudding project, it's still: "No."

Genomikon does have glowing smelly things which are growable for a
school pudding project for a Homeworker, and not thermosensitive unless
one counts 'cell death' as being sensitive which is unlikely.

## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################

On 1/28/15 5:55 AM, Cathal Garvey wrote:
> Actually, I think this is a *much* easier proposition than the
> melamine sensor (which never happened AFAIK). You can couple a gene
> system to heat-shock promoters, and you're mostly there.
>
> The complication is that the luciferin molecules are, I think, pretty
> heat-labile. So, it might not work simply because the luciferin is
> being pasteurised prior to oxidation by luciferase.
>
> Also, some "heat" shock systems are actually just "shock" systems, and
> may also activate under colder-than-ideal growth temperatures, so you
> might find that what you really have is "glows when not at exactly 37C".
>
> Covering for leaky expression would be important, but not that
> important. As long as the luciferin/ase production is at the end of
> the regulatory cascade and it scales nicely to input, some leaky
> expression will be pretty invisible.
>
> On 28/01/15 09:35, Jonathan Cline wrote:
>>
>> On Thursday, January 22, 2015 at 2:46:49 AM UTC-8, Lukas wrote:
>>
>> Hey Guys
>>
>> I want to create a thermosensitive yoghurt which reacts with
>> bioluminescence under heat. I have the model of Meredith Patterson
>> who has created a test for Melamin. I want to create the pudding for
>> a school Project.
>>
>> Lg Lukas
>>
>> BTW if somebody has time : can you list the equipment for the
>> experiment?
>>
>>
>> It will take you years to develop this, if it ever works at all, though
>> by the time you are done, you might have a Ph D. The equipment list is
>> partially in the DIYbio FAQ http://openwetware.org/wiki/DIYbio/FAQ
>>
>> You probably want to look into Genomikon.
>>
>>
>> ## Jonathan Cline
>> ## jcline@ieee.org
>> ## Mobile: +1-805-617-0223
>> ########################

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/54C93A3B.6040703%40ieee.org.
For more options, visit https://groups.google.com/d/optout.