For normal (not infinity corrected) objectives, things are really easy.
Remove the eyepiece from the microscope.
Where the eyepiece was, the light will project onto anything -- a piece of paper, the ceiling, a camera sensor without the camera lens. It is a real image.
The objective lenses are designed to work with a particular microscope tube length -- usually 160 millimeters. At distances above or below this, you get slight distortions that are easily corrected in software, but are often not noticeable anyway.
The farther away the sensor is from the objective, the more magnification you get. For most sensors, the actual resolution is larger than the size of the pixels in the sensor. When you look at the resulting image pixel-for-pixel on a computer screen, it will not look sharp. That is OK -- you just have more pixels than you need. So even a cheap low-resolution camera sensor can get you 250 nanometer resolution, which is close to the limit of your objective lens.
Infinity corrected objectives need another lens in order to form an image.
This is located inside the microscope tube. So even with infinity corrected objectives, you can remove the eyepiece and project a real image onto your sensor, projection screen, or ceiling.
On Sun, Feb 1, 2015 at 4:54 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>
> On Sunday, February 1, 2015 at 7:24:11 PM UTC-5, Nathan McCorkle wrote:
>> So how do we mount these properly to microscopes now? The larger lens
>> version says it has a 'CS' camera mount.
On Sun, Feb 1, 2015 at 4:30 PM, Cory Geesaman <cory@geesaman.com> wrote:
> The one I picked up has two mount holes - the topology of the PCB itself
> (solder points and such) mean you'll need standoffs or a custom fit piece if
> you want it mounted on metal. You could always do it cheaply with epoxy and
> some kind of scaffold to stick the epoxy to.
Hmm, I guess what always has confused me was how to adjust for
non-parallel image and sensor planes, as well as getting the right
height from the sensor relative to the mounting hole on the
microscope. And if a lens is needed, how to figure out which one, and
where to buy it with the most-compatible/easiest-to-modify mount.
Maybe this is something you just have to buy and iteratively
rig-things-up until you figure out a good design.
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