I used the columns that you can fill yourself with the HPLC machine. Don't know how much does it cost though. For DNA and some bigger stuff, I think I used Sepharose and Agilent used to have the whole range that you can choose from depending on what resolution you need. Regarding the dye labeling, I actually meant the covalently modified DNA. I used that before and of course that is stable; I admit that would be expensive though. Typically people run stuff sequentially and compare times but there is a variation from run to run, so it is more reliable and faster if you can do it in just one run.
-- Huge pressure is a bummer for HPLC. If we could do it in gas phase, it would be lower pressure for sure, less adsorption to the column material too and possibly faster (that would reduce the resolution though). I know that they gas chromatography is used for volatile stuff but I am puzzled why it could make it work for lyophilized stuff for example.
Where did you get HPLC machine? They look pretty expensive on Ebay.
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