The fact that it doesn't seems to integrate is clearly interesting. I did some work on plasmid integration and it is dependent on DNA repair (NHEJ). For integration to happens, the plasmid needs to get linearized and be close to a genomic double strand break for the NHEJ to integrate the plasmid. This also explains why plasmid integration is not an efficient process (< 1%) since linearized DNA is prone to recircularization by the NHEJ or is degraded.
-- http://www.nature.com/gt/journal/v10/n21/full/3302074a.html
For the cross-over, I was thinking about the human promoter region which might be similar to some endogenous promoter. I must say after that I don't really know the DNA size and similarity which is required for a cross-over to happen. But since we are talking about organisms which contains billions of cells, even an unlikely event could happen (after that, an integration/cross-over is not obligatorily deleterious).
On Sunday, 29 March 2015 23:37:54 UTC+1, Mega [Andreas Stuermer] wrote:
On Sunday, 29 March 2015 23:37:54 UTC+1, Mega [Andreas Stuermer] wrote:
For some reason the episome does not seem to integrate. At least it was described. Probably it is recognized as a chromosome which also won't integrate into another chromosome (hopefully).
Cross-over needs some homology right? Else it would happen between native chromosomes too extremely frequently?
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