Re: [DIYbio] Re: Phosphorescent PCR dye

On 05/06/2015 11:16 AM, Jeswin wrote:
> you might have to figure out
> how to quench the emission signal before the next collection point.

Not if you go slower than. He's thinking up another way, not how to
"be like" the current industry standard.

Slow, cheap and automated running a queue of samples could be very useful.

By slow, I am thinking of phosphors as used in analog oscilloscopes That put out light for
many milliseconds after a microseconds pulse of input light. Not minutes, not nanoseconds either.

Phosphors that put out for 10 seconds would be quite useful in an automated queue...
and if doing PCR, under the cycle time. Faster than the cycle time is not more useful.

Finding a phosphor that is compatible with and sticks to DNA is where to do some thinking and researching
if you want to figure out a "Phosphorescent PCR dye" method.

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