[DIYbio] Re: Something is Rotten in Denmark

3:50 PM |

I am the person that organised the event. I put tons of work and it and frankly I am super dissapointed to be accused of such things that we never ever stated. What made you even think we "are supposed" to eat it?

The vaghurt journey was a hard-working amazing project and we took all the measures to ensure the safety of the participants. Did you even see the inactivation of the biomaterials in the program?

Anyway, if anyone has nice questions about the project, please ask. But do not accuse or make a fuss without proper prior informing.

On Tuesday, June 30, 2015 at 6:33:31 AM UTC+2, Roninlaw wrote:

VAGHURT - DIY Vagina Yoghurt - Check the event and sign-up http://biologigaragen.org/2015/124

Seems like you could easily culture a pathogen. Michael Douglas would not approve. Anyone want to weigh in on the safety aspects? Today is the day they were supposed to eat it. I would urge you not to.

Dan

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[DIYbio] Re: Something is Rotten in Denmark

3:45 PM |

I am the person that organised the event. We put tons of work into it and I am terribly disappointed that you make wrong assumptions and came with such accusations. When and how did you get to the conclusion that we would eat it? We never stated that.

Please correct the post. I would really appreciate it. It feels really disappointing to assume that we are not scientific and we do not know the risks and we do not take the required measures. In the program it is stated that we will inactivate the bio materials.

Anyway, if anyone is interested in our journey, we will definitely like to share it. It was an amazing project.

On Tuesday, June 30, 2015 at 6:33:31 AM UTC+2, Roninlaw wrote:

VAGHURT - DIY Vagina Yoghurt - Check the event and sign-up http://biologigaragen.org/2015/124

Seems like you could easily culture a pathogen. Michael Douglas would not approve. Anyone want to weigh in on the safety aspects? Today is the day they were supposed to eat it. I would urge you not to.

Dan

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[DIYbio] Re: I want to Biohack! Thing is I just completed high school(A levels)

2:07 PM |

Hi Azra,

Welcome! None of us were born knowing how to do this. We bumbled along until we learned, just as you will do.

Do you have a project in mind? Or would you like to try some basic things to start with?

Stacy

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[DIYbio] Re: I have a plasmid I need from a paper- but I need help in having it constructed. Please help :)

2:05 PM |

Koeng is right; contact the author and ask. It would help to tell him what you want it for, and that you would share authorship on any paper that comes out of your work.

We call that process "clone by phone."

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Re: [DIYbio] Looking for Synthetic Bio startup partners

11:53 AM |

Hey Oobulu,
Sounds fascinating! If you can find cofounders by all means you're
welcome to apply to EU 2016 here: http://eu.indie.bio/apply-to-ireland/

If you'd like to talk more about the idea first you're welcome to
contact me off-list. :)

On 29/06/15 19:12, Oobulu Goobulu wrote:
> I'm looking for co-founders to apply to indie bio to develop functioning
> biological/electronic brain units that would co-exist with humans as
> companion cyborgs. Please reply or share if interested. Ideally these
> creatures will have synaptic nodes on part of skin surface to interface
> with electronic networks.
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Required || Network F5 consultant || Houston, TX || 6+ Months || Phone/Skype Hire

9:12 AM |

Dear Partner

Hope you are doing great!!

Please go through the requirement and let me know if you have any consultant for the same position.

Please do share me your hotlist also at Arpit@tresourceinc.com

Network F5 consultant
Houston, TX
6+ Months
Phone/Skype Hire

Here's the bullet points for the position down in Houston.
Locals are preferred but not required.
They really need someone with large enterprise experience whereas they have over 45k employees.

•    4-5 years F5 experience
•    1-2 year APM experience
•    iRules configuration experience

Thanks and Regards,
Arpit Arora | Sr.Technical Recruiter
Technology Resource Group Inc.
3736 Hillsdale Court Santa Clara, CA 95051
Office:408-709-1760 Ext : 961, Cell: 408-502-5132
Gtalk: Arpittechwire | Fax: 408-884-2409
Arpit@tresourceinc.com | www.tresourceinc.com

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Required || Citrix Support Engineer || Vernon Hills, IL || USC / GC

7:59 AM |

Dear Partner

Hope you are doing great!!

Please go through the requirement and let me know if you have any consultant for the same position.

Please do share me your hotlist also at Arpit@tresourceinc.com

Citrix Support Engineer

Vernon Hills, IL

6+ Months

USC / GC

3 -5 years of experience with more emphasis on the length of 5 years, seasoned with core experience (XenApps, Zen Desktop (would like beyond introduction level of Zen Desktop

Experience with 2003, 2008 etc OS Microsoft, 2012 is great.

Citrix core products – Mediframe, XenApp, Zen Desktop, Zen Server, Older versions and have progressed through them.

• Provide (Level 3) support for exiting Citrix systems involving XenApp and XenDesktop.

• Support & Experience with XenServer and Systems Hosted in a Virtual environment

• Support for Large Global deployments with XenApp and XenDesktop

• Provide design support for application processing requirements.

• Adept with large Citrix installations and deployments systems

• Provide technical support in engineered systems

• Manage activities of internal/external resources within the group or service delivery area.

• Support end-to-end Citrix connectivity.

Key Responsibilities

• Day-to-day technical and administrative support for XenApp 6.5 and XenDesktop 5.6 & 7.6 environments. (Level 2&3 )

• Handle high severity Citrix issues involving Regional and Global access.

• Manage daily work load through Remedy Ticketing system.

• Adhere to, and work within the confines of IT policies, standards and procedures.

• Develop, gather and measure relevant productivity and capacity metrics. (on Demand)

• Determine/manage priorities, timelines and schedules.

• Interact regularly with Team members & Customers to understand their business while anticipating their IT requirements.

• Support Service deliverables and develop improvement plans as needed.

• Support Global operational teams with all Citrix related issues.

• Support and Facilitate strategic global Infrastructure/Services initiatives.

• Strong cross-functional communications and interpersonal skills.

• Participate in periodic on-call Rotation

Successful Candidate will be required to submit to a National background Check and Drug Screen once confirmed for the Project

Thanks and Regards,

Arpit Arora | Sr.Technical Recruiter

Technology Resource Group Inc.

3736 Hillsdale Court Santa Clara, CA 95051

Office:408-709-1760 Ext : 961, Cell: 408-502-5132

Gtalk: Arpittechwire | Fax: 408-884-2409

Arpit@tresourceinc.com | www.tresourceinc.com

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Urgent Client Need: QA Automation Tester; Bedford, MA; 6+months

7:33 AM |

Hi,

Please find the below requirement and respond back to me at niranjan.kumar@vision3solutions.com/ 678-554-4785

Role: QA Automation Tester

Location: Bedford, MA

Duration: 6+months Contract

Must do an in-person interview.

Only Locals

Description:

Need a QA tester for 5-7 years of experience some one near to MA

Look for automation experience in the resume i.e. the candidate who can automate an existing test plan in XML and Web Services.

Thanks& Regards

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

niruk5503@gmail.com

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Fwd: Urgent Client Need: QA Automation Tester; Bedford, MA; 6+months

7:32 AM |

Hi,

Please find the below requirement and respond back to me at niranjan.kumar@vision3solutions.com/ 678-554-4785

Role: QA Automation Tester

Location: Bedford, MA

Duration: 6+months Contract

Must do an in-person interview.

Only Locals

Description:

Need a QA tester for 5-7 years of experience some one near to MA

Look for automation experience in the resume i.e. the candidate who can automate an existing test plan in XML and Web Services.

Thanks& Regards

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

niruk5503@gmail.com

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Fwd: Urgent Client Need: QA Automation Tester; Bedford, MA; 6+months

6:12 AM |

Hi,

Please find the below requirement and respond back to me at niranjan.kumar@vision3solutions.com/ 678-554-4785

Role: QA Automation Tester

Location: Bedford, MA

Duration: 6+months Contract

Must do an in-person interview.

Only Locals

Description:

Need a QA tester for 5-7 years of experience some one near to MA

Look for automation experience in the resume i.e. the candidate who can automate an existing test plan in XML and Web Services.

Thanks& Regards

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

niruk5503@gmail.com

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Fwd: Urgent Client Need: QA Automation Tester; Bedford, MA; 6+months

6:08 AM |

Hi,

Please find the below requirement and respond back to me at niranjan.kumar@vision3solutions.com/ 678-554-4785

Role: QA Automation Tester

Location: Bedford, MA

Duration: 6+months Contract

Must do an in-person interview.

Only Locals

Description:

Need a QA tester for 5-7 years of experience some one near to MA

Look for automation experience in the resume i.e. the candidate who can automate an existing test plan in XML and Web Services.

Thanks& Regards

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

niruk5503@gmail.com

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Re: [DIYbio] Re: Something is Rotten in Denmark

4:19 AM |

First of all, whenever you isolate an unknown organism from an environmental sample, you really should be doing that in a BioSafety Level 2 lab...

On the other hand, fermenting grapes to make wine, or making sauerkraut (beware, wild lactobacilli!) is also culturing unknown organisms from environmental sample... Yes, using a sample from humans makes it orders of magnitude more dangerous, but still, I would be very surprised if something bad happened. Yoghurt is not a good environment for typical pathogens.

Still, of course, not lege artis.

Jakub Friedl

On Tue, Jun 30, 2015 at 10:53 AM, Patrik D'haeseleer <patrikd@gmail.com> wrote:

Yes, this is definitely a really bad idea. Not that you couldn't do what they claim to be doing. But the way they're doing it isn't even close to safe. At least, it sounds like nobody is actually going to *eat* this vaghurt, since the program ends with "Visual inspection" and "Inactivating the biomaterials".

First of all, whenever you isolate an unknown organism from an environmental sample, you really should be doing that in a BioSafety Level 2 lab, because there is always some chance you'll be isolating (and greatly amplifying!) a pathogen instead. Check out the answer to my question to ask.diybio.org on this issue:

Under what conditions can I generate bacterial isolates from environmental samples in a BSL-1 environment?
Secondly, the fact that they're starting from a human sample increases the odds of isolating a pathogen by orders of magnitude. Sure, by culturing in milk they will be selecting for the more harmless lactobacilli. But they're only doing a single day of culturing, and they're not even picking colonies. So there's no chance in hell they'll get rid of anything that may be dangerous. Remember: one person's commensal bacteria is another's pathogens! Nice cup of Candida or STD yoghurt, anyone?

And even if they were able to select a single Lactobacillus strain (which they can't - not with the procedure they described), that's still not a guarantee it would be harmless. We tend to equate Lactobacilli with yoghurt, cheese and other fermented dairy products, but there's plenty of pathogenic ones as well:

Pathogenic potential of lactobacilli.
Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases.

You want to do this safely? You do the isolation in a BSL-2 lab, or at least a biosafety cabinet. You isolate colonies and subculture a couple times until you're confident they're axenic. They you use 16S sequencing to figure out what you have, and whether it's closely related to any pathogens. Then you try it *yourself* first, before you start exposing anyone else.

Good thing they're not actually planning on eating this...

On Monday, June 29, 2015 at 9:33:31 PM UTC-7, Roninlaw wrote:

VAGHURT - DIY Vagina Yoghurt - Check the event and sign-up http://biologigaragen.org/2015/124

Seems like you could easily culture a pathogen. Michael Douglas would not approve. Anyone want to weigh in on the safety aspects? Today is the day they were supposed to eat it. I would urge you not to.

Dan

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[DIYbio] Re: Something is Rotten in Denmark

1:53 AM |

Yes, this is definitely a really bad idea. Not that you couldn't do what they claim to be doing. But the way they're doing it isn't even close to safe. At least, it sounds like nobody is actually going to *eat* this vaghurt, since the program ends with "Visual inspection" and "Inactivating the biomaterials".

First of all, whenever you isolate an unknown organism from an environmental sample, you really should be doing that in a BioSafety Level 2 lab, because there is always some chance you'll be isolating (and greatly amplifying!) a pathogen instead. Check out the answer to my question to ask.diybio.org on this issue:

Under what conditions can I generate bacterial isolates from environmental samples in a BSL-1 environment?

Secondly, the fact that they're starting from a human sample increases the odds of isolating a pathogen by orders of magnitude. Sure, by culturing in milk they will be selecting for the more harmless lactobacilli. But they're only doing a single day of culturing, and they're not even picking colonies. So there's no chance in hell they'll get rid of anything that may be dangerous. Remember: one person's commensal bacteria is another's pathogens! Nice cup of Candida or STD yoghurt, anyone?

And even if they were able to select a single Lactobacillus strain (which they can't - not with the procedure they described), that's still not a guarantee it would be harmless. We tend to equate Lactobacilli with yoghurt, cheese and other fermented dairy products, but there's plenty of pathogenic ones as well:

Pathogenic potential of lactobacilli.
Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases.

You want to do this safely? You do the isolation in a BSL-2 lab, or at least a biosafety cabinet. You isolate colonies and subculture a couple times until you're confident they're axenic. They you use 16S sequencing to figure out what you have, and whether it's closely related to any pathogens. Then you try it *yourself* first, before you start exposing anyone else.

Good thing they're not actually planning on eating this...

On Monday, June 29, 2015 at 9:33:31 PM UTC-7, Roninlaw wrote:

VAGHURT - DIY Vagina Yoghurt - Check the event and sign-up http://biologigaragen.org/2015/124

Seems like you could easily culture a pathogen. Michael Douglas would not approve. Anyone want to weigh in on the safety aspects? Today is the day they were supposed to eat it. I would urge you not to.

Dan

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Re: [DIYbio] Community Lab in Belgium

10:02 PM |

Thank you! We are in Ghent. We've found a location we can use with the necessary tools to build stuff, it's great.

On Tuesday, June 30, 2015 at 1:37:14 AM UTC+2, mlp wrote:

Welcome to the list! There are quite a few folks here in Belgium, actually, many in Brussels. Where are you based?

Cheers,
--mlp

On Wed, Jun 24, 2015 at 7:40 AM, Winnie Poncelet <winniep...@gmail.com> wrote:
Hello,

we are a group of biochemists starting a public Biolab in Belgium. We are currently building basic equipment and talking with potential partners.

Is there anyone here who would like to be involved in this project?

Regards
Winnie

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[DIYbio] Something is Rotten in Denmark

9:33 PM |

VAGHURT - DIY Vagina Yoghurt - Check the event and sign-up http://biologigaragen.org/2015/124

Seems like you could easily culture a pathogen. Michael Douglas would not approve. Anyone want to weigh in on the safety aspects? Today is the day they were supposed to eat it. I would urge you not to.

Dan

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[DIYbio] Re: I have a plasmid I need from a paper- but I need help in having it constructed. Please help :)

8:58 PM |

You can create it yourself, but it's pretty much exponentially easier to just ask for the plasmid. Unless you want the task of painfully recloning a plasmid, I would recommend you do that.

-Koeng

On Monday, June 29, 2015 at 3:35:04 PM UTC-7, GFP4Me wrote:

Hi,

I'm looking to use the exact plasmid as written in a paper ... http://www.sciencedirect.com/science/article/pii/S108718450400180X

But my genetics background is fairly limited. The plasmid is written out in the paper, but I am looking to be able to have it possibly created for me. I'm looking for someone to help me get to the point where I can purchase it online.

The plasmid is referred to in the paper as pGR4-4iGM3' , and also as pUGiGM3'.

Thanks for any responses,
GFP4Me

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Re: [DIYbio] Community Lab in Belgium

4:36 PM |

Welcome to the list! There are quite a few folks here in Belgium, actually, many in Brussels. Where are you based?

Cheers,

--mlp

On Wed, Jun 24, 2015 at 7:40 AM, Winnie Poncelet <winnieponcelet@gmail.com> wrote:

Hello,

we are a group of biochemists starting a public Biolab in Belgium. We are currently building basic equipment and talking with potential partners.

Is there anyone here who would like to be involved in this project?

Regards
Winnie

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[DIYbio] I want to Biohack! Thing is I just completed high school(A levels)

4:07 PM |

What do you have in mind? Growing bacteria, or actually genetically engineering them?

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[DIYbio] I have a plasmid I need from a paper- but I need help in having it constructed. Please help :)

4:03 PM |

If you have the sequence, you can have it synthesized for ~200$ to 800$ (depending, if you want the promoters and terminators synthesized alomg with it)

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Re: [DIYbio] Can anyone tell me what kind of equipment and supplies I would need to look at telomeres in cells?

3:59 PM |

On 06/23/2015 07:50 PM, Brad S wrote:
> Can anyone tell me what kind of equipment and supplies I would need to look
> at telomeres in cells?
>
> This would be to test different substances on the cells to try and activate
> telomerase.
>

You should take that question to the GRG list - there are several
frequent posters there who are involved in exactly this or similar
ventures, and will probably be able to give you some guidance.

http://lists.ucla.edu/cgi-bin/mailman/listinfo/grg

Reason

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Required || Application Packager || Tarrytown,NY || Phone Hire

2:00 PM |

Dear Partner

Hope you are doing great!!

Please go through the requirement and let me know if you have any consultant for the same position.

Please do share me your hotlist also at Arpit@tresourceinc.com

Application Packager

Tarrytown,NY

Phone Hire

Job Description
Implement software installation packages and supporting deployment infrastructure on a distributed network for a new product under development.

Candidates for this position should have the following skills:
• 1+ years developing software installation packages
• Working knowledge Windows Installer Technology
• 1+ years C# or WiX (Windows Installer XML) tool set

The following skills would be beneficial but are not mandatory:
• PowerShell scripting

Thanks and Regards,

Arpit Arora | Sr.Technical Recruiter

Technology Resource Group Inc.
3736 Hillsdale Court Santa Clara, CA 95051

Office:408-709-1760 Ext : 961, Cell: 408-502-5132

Gtalk: Arpittechwire | Fax: 408-884-2409

Arpit@tresourceinc.com | www.tresourceinc.com

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[DIYbio] A year at -20... does it work?

12:51 PM |

Hey guys

A year ago I began an experiment to see how I could store things long term in a DIY manner. The results (note: poor controls. Please don't sue me and take all of this as opinion)

-Plasmids are best stored in E coli stabs

-E coli strains are best stored at -20 at 40% glycerol

Simply put, I put 3 things in the freezer for the year: bacteria culture with 10% glycerol, 20% glycerol, and 40% glycerol. I also put in a stab where I just picked a colony and stabbed it into a tube, normal method.

For the 10%, even though there was clear settling of cells I scraped from the top because the culture was frozen. No colonies appeared on the plate. I did the same with 20% and 1 colony appeared. 40% was not frozen so I just took a tiny from the bottom as well when I streaked and got surprising results

Although I used 1/5 of the small pellet at the bottom, I got *significantly* more colonies than I got with streaking out the entire stab. However, nearly 1/3 to 1/2 of these cells no longer held the RFP plasmid even though I plated on amp. On the stab plate, however (same batch of plates), I did not get a single white colony. There were less colonies, but still about 50 colonies.

So, overall, if you wanna store plasmids without an expensive freezer, store them as stab cultures. I can vouch that it works. (however sometimes mutations can occur in the genomes of the cells in stab cultures http://www.ncbi.nlm.nih.gov/pmc/articles/PMC516597/ )

-Koeng

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[DIYbio] Looking for Synthetic Bio startup partners

11:12 AM |

I'm looking for co-founders to apply to indie bio to develop functioning biological/electronic brain units that would co-exist with humans as companion cyborgs. Please reply or share if interested. Ideally these creatures will have synaptic nodes on part of skin surface to interface with electronic networks.

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Required || QA tester with E-commerce || Ann Arbor, MI || Long Term

9:20 AM |

Hope you are doing great!!

Please go through the requirement and let me know if you have any consultant for the same position.

Please do share me your hotlist also at Arpit@tresourceinc.com

QA Tester

Long Term Contract

Ann Arbor MI

E-Commerce is a MUST HAVE

Summary: The Quality Control Analyst is responsible for overall quality assurance throughout the application development cycle for the eCommerce group. Through the leadership provided by the Quality Control Manager, the Quality Control Associate will partner with the development team for overall code quality.

GENERAL RESPONSIBILITIES

· Analyze the product features, and the product as a whole, to ensure it behaves as intended

· Ensure that components and features are complete based on the department definition of "done"

· Participate in developing and documenting User Stories, including testing estimates and validation tests

· Write the test cases/scripts – ideally up-front but on a just-in-time basis per feature

· Partner with the developers to ensure all areas of the application are fully tested and issues are clearly logged and prioritized consistently

· Manage the defect log and ensure issues are resolved, including validation of the fix

· Analyze and determine proper testing procedures/processes for testing both hardware and software.

· Participate in developer integration, system integration, and acceptance testing of all eCommerce applications

· Certify software functionality for new Production releases

· Provide exploratory testing to understand the feature and how it is implemented, to find additional or unexpected behavior, and to refine and define additional test cases for the feature

· Ensure all non-functional requirements (performance, scalability, security, usability, etc.) are met

· Act as the entry point for the Help Desk to provide an initial review and assessment of reported defects

· Inform QC Manager of testing status

QUALIFICATIONS

· Bachelor's degree in computer science or equivalent experience

· Ability to work independently or in a team environment

· Excellent attention to detail

· Excellent communication skills

· Hands-on experience with Mobile applications (iOS and Android)

· Hands-on experience with eCommerce web sites

· Basic understanding of the software development lifecycle

· Familiarity with common development tools, such as JavaScript, JSP, AJAX, CSS, XHTML, Flash, browsers (IE, Firefox, Safari, etc.), SQL, Unix, Linux, Apache, Tomcat, IIS, MySQL, Perl

Thanks and Regards,

Arpit Arora | Sr.Technical Recruiter

Technology Resource Group Inc.
3736 Hillsdale Court Santa Clara, CA 95051

Office:408-709-1760 Ext : 961, Cell: 408-502-5132

Gtalk: Arpittechwire | Fax: 408-884-2409

Arpit@tresourceinc.com | www.tresourceinc.com

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