A simpler approach is to use a android or apple tablet as an illuminator (many apps that provide bright RGBW colors, I use a free app called "Lightbox"). Place the plate on top of the tablet and then take a picture through an app, such as "ELISA Plate Reader" on Android. The App will then give you values for a 96 well plate. Enzo also has an ELISA reader app. You can also determine HSV values through most photo processing software such as GIMP or JImage. Jiamge already has a module for quantitation of grid images such as 96 well plates. For the photo softwares you just need to take an image through a regular camera and import into the software.
-- let me know if you need more details.
Thanks
Abizar
On Saturday, August 29, 2009 at 4:34:38 AM UTC-7, Inventoriffic wrote:
On Saturday, August 29, 2009 at 4:34:38 AM UTC-7, Inventoriffic wrote:
Hi,
I am planning on building a microplate reader for some experiments I
have planned. I'm just going to outline my plan to see if others have
some better ideas, or see folly where I don't :). It's been many years
since I was in biotech and I never went into the hardware side before.
I am using an arduino based platform for this.
1. Proof of concept Build basic prototype spectrophotometer
- insert curvette's manually to switch between sample & controls
- basic LDR + LED combo
- no wavelength filter
2. Programmatic wavelength alteration for the basic spec
- test a laser based light source
- use a diffraction grating + servo to alter the wavelength of the
light source
At this point I will test the accuracy at a local lab.
3. Build prototype stepper motor based plate reader
- designed so that I have control over x-y position + wavelength
My major question is around finding a good diffraction grating (or
alternative wavelength modifier) that I can control easily to get the
desired output wavelength. Any ideas? All criticism is helpful :)
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