[DIYbio] Re: I want to Biohack! Thing is I just completed high school(A levels)

Plants, sure. I'd also put focus to further defining "resilience." start with one gene (whether that be for heavy metal resistance, salt tolerance, etc.) and build from there. Really define what sort of traits you would like to express in the plants and for what function. from there read read read!! Build yourself a 'library' of genes you come across while reading papers on crop resilience. ... And keep your mind open to what you're reading and don't be afraid to delineate to other aspects of biology or transformations. 

I wouldn't put the cap on what plant to work on quite yet... before you make any decisions or start experiments do your homework. You'll probably find that you want to first get experience with model organisms for transformations, I'd think maize or tobacco because they have been extensively studied. Start off wetlab work with proven protocols and prove to yourself that you can first replicate other's work before starting your own experiments. Design 'em as you go along, but you'll see the ideas take more specific shape the longer you've been in it. Check out http://diybio.org/local/ to see if there is a hackerspace around you. plug in with the existing projects and learn the basics, and be patient! Ask for help and you'll probably get it.

Look into plant transformations, golden braid cloning, and agrobacteria mediated transformations.. there are papers out there on phytoremediaiton that could give you interesting ideas into tolerance of condition XYZ.. Also familiarize yourself with the concepts of PCR, primers, restriction enzymes, the tools and machines you'll need (autoclave, centerfuge, incubators, shakers, water baths, spectrophotometers, etc.) or ways you can get around using them or even build your own. 

On Monday, July 20, 2015 at 8:57:24 PM UTC-7, Kit Martens wrote:
Project idea:

Create a more resilient grain plant with all the essential amino acids and make it available to the public. With companies like monsanto cornering the market with their seeds and taking advantage of farmers, something needs to be done. Perhaps engineering a better grain plant for free and making it available is a good solution. THe first thing we'd need to do is agree on the plant we're going to work on. 

On Monday, June 29, 2015 at 3:34:50 PM UTC-7, Azra Abdi wrote:
I want to do biomedical engineering and this whole biohack thing is just what I was looking for. Thing is I just finished high school, I can afford some of the equipment using cash from my part time job but my problem is that  you all seem to be degree holders and know what you're doing, so was hoping someone could help me get started. There are no biohackers that I know of here in kenya and was thinking of involving a classmate of mine to help.

Thanks

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