[DIYbio] Re: Looking for a gene to start with when doing PCR

Hi, 

ACTN3, for example

take a look

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0043132#s2

With a PCR you just go for an insdel site. easy to see with sybr

example = C/T CAT->TAG QLN->STOP

Also you can look for STR sites

If you are looking for the Rs72921001 (http://snpedia.com/index.php/Rs72921001), you'll need to use a qPCR or Sanger. With a "normal/regular" electrophoresis you cant distinguish nucleotides. 

El viernes, 24 de julio de 2015, 16:14:17 (UTC+2), Sina Amoor Pour escribió:

Hello,

I am one of the organizer of Stockholm Makerspace. During the spring we moved to a new location and got the opportunity to build a wet lab. Through a network of people and a lot donations from life science companies, universities and cooperation with the local iGEM team we have managed to build a lab with all the equipment needed for at least PCR, transformation of bacteria and simple light microscopy. Now we are in talks with a couple of companies selling reagents and primers. All that is needed now for us to do our first PCR experiments is a gene that we can amplify. I'm looking for a gene that would be fun for people that have never been in a lab to amplify and look at with gel electrophoresis.

I have been thinking about doing the cilantro test. But I'm not sure which gene to test for. Is it the OR6A2 gene that should be amplified? And if you have it you should hate cilantro and if you don't have it you should love cilantro (bascially)? If not, which gene is the cilantro-hating gene?

And do you have any other suggestion for other fun genes that we can try out?

All suggestions and advices are appreciated!

Thanks,

Sina