Well i am not disputing you - but in my case the species i am working with lacks well defined polymerase III promoters. Therefore am more interested in methods which would function with pol II promoters. For obvious reasons i dont expect transcription of tRNA by a pol II would function as intended in a eukaryotic cell.
-- But can anyone point to a couple of articles substantiation the use of AT rich spacers between the ribozymes?
Thanks
Den mandag den 23. november 2015 kl. 11.00.52 UTC-8 skrev Mega [Andreas Stuermer]:
Den mandag den 23. november 2015 kl. 11.00.52 UTC-8 skrev Mega [Andreas Stuermer]:
But AFAIK it doesn't matter whether it uses polIII or polII. The tRNA processing site is cut, so it cuts away the 5' cap and the polyA tail. Without these features the RNA is not exported from the nucleus into the cytosol. RNA is RNA, doen't make a difference then?
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