Re: [DIYbio] Re: E. coli chromossome integration

Why such scrutiny over placement of a gene? Do you have your genes flanked by homology with E. coli chromosome?
Some intergenic regions have quirky DNA. Maybe you could use that to your advantage.
If you are useing NHEJ, make sure you are working with a strain that's expressing gam homodimers, or bacterial Ku homo/heterodimers. That should make things easier even with liniarized DNA.
Alternatively there are intergrases you could use.

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