I am somewhat confused. Being new to PCR and electrophoresis I am mixing up TAE in 50-1 strength with Glacial Acetic acid and EDTA added in correct proportions and finding the end pH to be far more acidic than it is documented on most sites. Some sites say do not test your mix after making up TAE and just make sure the mix is correct.
-- Others say it must get to 8.3, well I tried adding NaOh 1M and found that I seemed to need a great deal to bring it close to 8.3 and have actually run through my current supply of NaOh.
How much of an issue is getting this pH to around 8.0??? I would welcome some suggestions.
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