I don't know where you live, but you should look into local colleges or universities and see if there are any PI's there working on similar things. If approached in the right way, and shown a decent proposal that looks like it's backed with scientific understanding and literature references, I've known some to being open to taking in research assistants. You might even end up getting a stipend and the PI might pay for some research expenses. Most importantly, you'd be in a properly equipped lab to do the things you're talking about.
On Fri, Jan 15, 2016 at 8:40 PM, coolcash2004 <davidconwayj@gmail.com> wrote:
Would anyone be willing to help me order and prepare Borrelia bacteria samples? We would need to order the samples and do subculture / antibiotic treatment to turn them into persister cells. You would need to confirm that they have entered the persister state. You would then prepare the samples to be sent out for proteomic profiling. You would follow the existing process for subculture / antibiotic treatment to ensure that they have entered persister state (several studies have done this process and it is documented - the cells reach persister state in 10 days). We would also be in contact with the research center doing the proteomics.--Please let me know if you would be interested and name your price. I am young (20s), broke, and working independently, so the cheaper the better. I am trying to develop a cure for persister lyme disease.Before beginning any work I am hoping to learn:-How much would you charge to do this?-What would be the additional cost of the actual bacteria / etc that we would need to order?-Is it possible to order human nerve tissue and grow subculture on that tissue? If yes, how much more would this cost?-Is there a better way to do this?-What information would you need from me before we start talking?I'm hoping to find someone that I could potentially work with in the future as well - someone who would be interested in providing guidance on the project as we progress would also be helpful.Thanks,
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