Streptomyces chop off massive pieces of their DNA at random and shuffle things constantly. Transforming them is terrible. Plasmid loss is common. Integrated DNA loss is common.
On Thu, Jan 14, 2016 at 11:22 PM, Matt Lawes <matt@insysx.com> wrote:
How about something like Streptomyces or Myxobacteria? They do well around 28-30C but really hate mid / high 30s.
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On Jan 14, 2016 11:20 PM, Koeng <koeng101@gmail.com> wrote:
Possibly program a temperature sensitive lambda red system with a positive feedback system loop connected to a toxin. There are varieties which repress at 30 but not at higher temperatures, like 37. Don't know if you could get as specific as 33c.--
-Koeng
On Wednesday, January 13, 2016 at 4:26:04 PM UTC-8, Antony Evans wrote:Hello,
I'm looking for a bacteria strain which is amenable to transformation and can't grow at 33 degrees Celsius (so that it can't grow in warm blooded animals).
Any suggestions?
Antony
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