Re: [DIYbio] What do open source PCRs need?

On 02/02/2016 11:41 AM, Mac Cowell wrote:
> It was hard to determine the temperature of the mastermix in the tube. We tried embedding a thermistor in a similar mass at the
> bottom of a tube, but it was really fiddly. It seemed that the devil was in the details of knowing what particular temperature the
> tubes were at.

that is a clue. Plastic is a heat lagger and also a heat sink with high heat capacity.
Changing to glass tubes would help. My kickstarter for an empty radio enclosure is not
inspiring maker hackers to instrument their back yards yet...

How many on this list would want a fast ramping air PCR thermocycler that needs no lid because it also
shakes the contents, (which also contributes to ramp rate performance)?

It could cost $190. On rev. 2 it might cost $120 and come with wifi webserving interface, python serial/USB port,
SD card for logging, 24VDC power port and wall wart power converter, and take up 10cm x 10cm bench space.

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