Now I'm interested what the status of DIY electroporators is?
On Wednesday, April 27, 2016 at 7:48:37 PM UTC-7, Marc Juul wrote:
-- Saw some talk of ESP8266 and other ideas floating around. I've done a uC controlled flyback cap charger before, so I'm wondering what the actual technical difficulties are that remain?
Charge a cap to a couple kV, then switch it to the cell for the proper length of time with an IGBT? Anything I'm missing?
On Wednesday, April 27, 2016 at 7:48:37 PM UTC-7, Marc Juul wrote:
If no designs exist, who would be interested in having such a device and what are your use-cases / organisms of interest?It looks to me like it should be simple enough to build one for <$100.I've found old threads on this list where people are discussing DIY electroporators but it doesn't seem like anyone published a new design since this expensive and impractical one from 1991:Has anyone made anything better?
http://www.biosci.missouri.edu/smithgp/ PhageDisplayWebsite/ electroporator.doc
Do biohackers actually use electroporation and if not, why not? What is your preferred transformation protocol for the organisms you work with.marc/juul
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