Re: [DIYbio] Re: low cost closed electroporator

Yes.  I've got more experience with electronics now too.  I've been using the Biorad a bit lately, but might have a chance to crack it open again.  I've got my eyes open for an old unit now though, so hopefully I can fine one of those vintage units that are just some caps and switches with no real electronics to speak of.

Kind of interested in the flyback designs that are being pursued.  I made a flyback circuit to an ignition that was quasi-resonant with ZVS, completely controlled by a PIC.  I never mastered the calculations or really the theory, but just overcame that by directly controlling the switching with a uC.


On Thursday, May 5, 2016 at 11:12:21 AM UTC-7, Nathan McCorkle wrote:

Jake, did you have a biorad micropulser? When you loaned me yours, years ago, I took it apart but I think failed to take photos, would be nice to get a look now that I'm more experienced with electronics.

On May 4, 2016 10:23 PM, "Jake" <jake...@mail.com> wrote:
We really should develop an open hardware unit.  They aren't that complicated.  Sadly, I've passed up a few old ones at auction that probably would have been dirt simple to reverse engineer. My current unit is a bit overcomplicated.


On Friday, April 29, 2016 at 2:09:50 PM UTC-7, Koeng wrote:
Hey

I have some organisms I would like to transform, and frankly, electroporation just works better. I would love to use an open source one, but that isn't happening very soon, so I have been considering for a while getting a used one off ebay. Does anyone have experience with a good pulse chamber + power supply combo? I would like to know what has been worked on (like perhaps the invitrogen electroporator ii) before I commit to a purchase.

-Koeng

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