Re: [DIYbio] Re: low cost closed electroporator

sorry if i sound arrogant. my background is more in the biology field than in the electronics

yes, you are right. this device is for mouse in utero electroporation, so it's normal that you use lower currents. but i guessed that it should be easier to start from this paper and the info from missouri university

El viernes, 6 de mayo de 2016, 10:15:38 (UTC+2), Marc Juul escribió:


On Fri, May 6, 2016 at 12:41 AM, Kermit Henson <kerm...@gmail.com> wrote:
there are papers with low cost (50usd) open source electroporators (see link and attached file). 
dont understand why we have to reinvent the wheel. i prefer to copy and improve the results

Well because I didn't know about it :) The electroporation voltage used in that paper was much lower than what I've ever seen used for e.g. yeast or E. coli and their device does not seem capable of producing higher voltages. Still, I'll be sure read it more thoroughly. Thanks for sharing! I definitely don't want to duplicate work that has already been done.
 

I did see that one but that design is $500 and requires an external 2.5kV supply. It should be possible to make a device capable of a wide range of voltages all the way up to 2.5kV for less than $100 total and without relying on an external supply.
 
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marc/juul

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