[DIYbio] A hypothetical protocol for DIYBIO DNA synthesis

As some of you may know a means of synthesising DNA that is accessible to the ambitious and well informed amateur has been a long term intellectual pursuit of mine. A kind of theoretical moby dick. Here I'd like to present something like a paper (for a certain value of paper) containing my latest thoughts. If these ideas pan out it would permit synthesis of DNA fragments potentially 100s of nucleotides long with out any pre made oligos (or at least only non gene specific ones), with out invoking potentially toxic and hard to obtain phosphoramidite based chemistry or any thing similar. A method based almost entirely on enzymatic processes. Of course there are a lot of ifs. However I'm not in a position at the moment to take the theory any further into actual experimentation. So I'm opening the idea up to the community in the hope that you can collectively make something of it. It's not really a better alternative for those of you who can already easily order DNA, it's not nearly as efficient as the phosphoramidite method not even in theory. It's a significantly more complex process. However for those of you who can't get synthesised DNA or who want to make your own as a project this may be of interest.