Thanks for the advice, everyone. I'm familiar with the differences in pellet visibility, but I didn't know about the difference in salt solubility. In any case, I'm not concerned with manual pelletting and washing, but spin column kits like this one: http://dongshengbio.com/en/xxcp.asp?id=271
fredag 22. juli 2016 05.45.32 UTC+2 skrev Nathan McCorkle følgende:
-- Best regards,
JP
fredag 22. juli 2016 05.45.32 UTC+2 skrev Nathan McCorkle følgende:
From the mini-prep folks:
https://www.qiagen.com/us/resources/molecular-biology- methods/dna/#Isopropanol
precipitation of DNA
Mentions better precip at warmer temps, better salt solubility (which
leads to harder to see glassy pellets rather than clouded-with-salt
pellets)... but the main point I remember: downstream processes could
be affected -- because isopropanol is less volatile it dries more
slowly or not completely if you are too fast... slower drying gives
more time for energy-state minimization, thus when it is dry it is
harder to redissolve (it must be denser and more closely-packed I
guess).
On Thu, Jul 21, 2016 at 5:07 PM, Cathal (Phone)
<cathal...@cathalgarvey.me> wrote:
> Isoprop is fine for washing minipreps. IIRC it has slightly different
> performance on RNA, and it will behave differently WRT salt also. But, for
> minipreps, it's not very important. You need a bit less of it than ethanol,
> it's a stronger precipitant.
>
> Warning: DNA pellets washed with ethanol tend to become white and easily
> visible, whereas with isoprop they can be glassy and transparent.
>
> On 22 July 2016 00:31:13 IST, Cory Tobin <cory....@gmail.com> wrote:
>>
>> I've used isopropanol for Qiagen minipreps before and it didn't make
>> any noticeable difference.
>>
>> -cory
>
>
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