Natto is fermented with B.subtilis natto, many or most strains of which carry one or two plasmids. The plasmids I'm familiar with are low copy number though so aim for high cell titers. Also, gram positive near-wildtypes means you'll need lysozyme!
I've never done it, but it might work. And not just BSL1 but foodgrade/GRAS. :)
On 21 July 2016 17:17:00 IST, jarlemag <jarle.pahr@gmail.com> wrote:
Hello,As part of setting up a DIY bio lab and introducing newcomers to molecular biology, it would be nice to demonstrate plasmid extraction and subsequenct study of the plasmid by restriction digestion or sequencing. The catch is, local regulations prevent us from working with genetically modified organisms, including organisms transformed with engineered plasmids. Are there any wild-type BSL1/generally recognized as safe organisms harboring natural plasmids which might be suitable? A strain that is readily available from a culture collection or from fellow DIY biologists would be preferable.Best regards,Jarle Pahr
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