https://www.scbt.com/scbt/whats-new/antibody-exchange-program
Maybe this would be an interestimg option/alternative/... ?
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[DIYbio] Have you raised monoclonal antibodies in your garage?
Rate $55/hr || Urgently looking for SAP FICO functional Consultant(Local) || Sunnyvale, CA
Dear Partner,
Hope you are doing great!!
Please go through the requirement and let me know if you are having any consultant for this position.
Please share me the profile asap as this is a very hot requirement.
SAP FICO functional Consultant
Sunnyvale, CA(Local)
6+ Months
CMT Level 9
CONFIGURATION EXPERIENCE IS REQUIRED (will be tested for during interview)
SAP functional experience specializing in design and configuration of SAP Finance modules. Experience in full implementation of SAP FI modules.
Experience in gathering business requirements, providing conceptual and detailed designs to meet business needs, performing necessary SAP configurations, writing detail specifications for development of custom programs, testing, co-ordination of transports to production and post go live support.
Requires excellent verbal and written communication skills, including presentations creation and delivery.
Minimum 5 years of SAP FICO / FI-CA experience. Good understanding of accounting concepts.
Bachelor's Degrees and Post graduate degree (MBA / CA) is required.
Prior experience in high tech or software industries is preferred
Start Time:12:00 AM
Hours:8:00am to 5:00pm
Best Regards,
Arpit Arora
Pyramid Consulting, Inc.
Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home
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Re: [DIYbio] AgroBacteria DIY BIO DALLAS
Sebastian S. Cocioba
Is it hard to get approved? Do I need the form each time? What strain should I get?--
On Monday, October 31, 2016 at 4:35:13 AM UTC-5, cory....@gmail.com wrote:-coryFor domestic transportation of Agrobacteria you need permission from USDA APHIS which you can request using form PPQ-526. Plan a month or so in advance because they're kind of slow.If you plan on transporting plants that were genetically transformed with Agro you'll need USDA APHIS form 2000. Although, Arabidopsis thaliana is exempt.
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Re: [DIYbio] AgroBacteria DIY BIO DALLAS
Sebastian S. Cocioba
I am trying to start a DIYBIO group here in Dallas at Dallas Maker Space--I am working on Plant Tissue Culture at the moment, I started looking into ordering AgroBacteria, but it seems I need a special licence to buy it across state lines.From what I have read the license is quite hard to get?
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Re: [DIYbio] AgroBacteria DIY BIO DALLAS
https://www.eauth.usda.gov/
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Required || Salesforce Developer || Sunnyvale, CA || 6 months Contract
Hope you are doing great!!
Please go through the requirement and let me know if you are having any consultant for this position.
Please share me the profile asap as this is a very hot requirement.
Salesforce Developer
Sunnyvale, CA
6 months Contract
Opportunity
We are seeking a highly motivated individual to work on its Business Applications group and help perform full lifecycle development of SalesforceCRM/Service Cloud. The individual will be responsible for Salesforce product enhancements and defect fixes based on requirements you gather from internal stakeholders to address their Salesforce-related needs. You will also be relied upon to integrate Salesforce with other applications.
Responsibilities:
- Drive and implement a merchandising process and build required solution in Salesforce
- Facilitate requirement gathering workshops, document, analyze and prioritize detailed business requirements based on users and business needs
- Translate high-level business and user requirements into functional requirements for design and development
- Own development, testing and delivery of native Salesforce components and Visual force components
- Scope and provide accurate work estimates for all deliverables
- Work with business users to develop use cases for testing of new functionality
- Lead system testing efforts, ensuring that issues are identified, tracked, reported on and resolved in a timely manner
Qualifications
- Bachelor's degree in Computer Science or related field; or equivalent work experience
- Proven experience with Salesforce development, integration, and bug fixes
- Experience in Apex, Visualforce, Javascript, and HTML/CSS.
- Experience creating SQL tables and queries.
- Strong technical, problem resolution and analytical skills; able to multi task and prioritize effectively
- Excellent verbal and written communication skills
Best Regards,
Arpit Arora
Pyramid Consulting, Inc.
Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home
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Re: [DIYbio] AgroBacteria DIY BIO DALLAS
On Monday, October 31, 2016 at 4:35:13 AM UTC-5, cory....@gmail.com wrote:
-coryFor domestic transportation of Agrobacteria you need permission from USDA APHIS which you can request using form PPQ-526. Plan a month or so in advance because they're kind of slow.If you plan on transporting plants that were genetically transformed with Agro you'll need USDA APHIS form 2000. Although, Arabidopsis thaliana is exempt.
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Re: [DIYbio] AgroBacteria DIY BIO DALLAS
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[DIYbio] AgroBacteria DIY BIO DALLAS
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Re: [DIYbio] Re: Biohacking Start Equipment
Ok thank for the Information
My own pcr works now.
The question is. IN German genetic modificaiton isnt allowed. But in some Countries crispr doesnt count as GMO. Do anyone know the laws about that in Germany?
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Re: [DIYbio] Re: Biohacking Start Equipment
- Adjustable pipettors (ranging from 1 ul to 1000 ul). Ebay a good source. Typically US$50-100 per piece. Chinese brands for about US$20.
- Thermocycler. Again ebay a good source. US$100-1000 for used systems. Good brands are Applied Biosystems, MJ Research, BioRad ... Likely that the Peltier elements will have to be replaced. Also possible to make your own. Simplest is to have a halogen lamp based heating with an arduino controlling lamp on and off based on feedback from a thermistor.
- Microcentrifuge. Easy to make your own or buy a used one.
- Gel box for agarose gel electrophoresis and power supply. Gel boxes very easy to make your own. Will need to buy platinum wire. power supplies - if you are familiar with electronics - not hard to build your own or buy a used one, US$50-100 on ebay.
- Transilluminator - again make or buy with UV LEDs or get a handheld UV lamp (fluorescent tubes or LEDs)
- PCR reagents. BioRad sells kits for educational use. http://www.carolina.com also has cheap supplies. $100/kit.
- Ice buckets. Any small styrofoam box painted with multiple coats of water based acrylic paint.
- Cold storage. Domestic freezer set at coldest temperature. Typically -20C. Dry-ice + isopropanol slurry for -80C.
- Colorimeter/fluorometer. Easy to do DIY with LEDs and photodiodes.
- Plastics - tubes, pipettes, holders, pipette tips, ...
- DNA/RNA extraction - something to grind tissues (blenders, homogenizers), DNA binding powders (alumina, silica powders for sand blasting washed with alkali and water), centrifuge and pipettors
- DNA/RNA quantitation - by gel electrophoresis/transilluminator, by UV light colorimeter (260 nm), by fluorescence (led fluorometer), pipettors
- DNA cloning - Incubator, water bath, dry bath, or thermocycler. Pipettor. Gel electrophoresis/transilluminator. Centrifuge. Pipettors.
- Growing microbes - incubator (warm box - DIY), shaker, test tubes or flasks or Starbucks Frappucino bottles. Red LED colorimeter to measure growth. Pressure cooker or microwave to sterilize.
Thanks for all the Answers.--I currently try to build my on PCR. The Question is now:
Which Primer and Polymeras do i need? Where can i buy the chemicals?
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Re: [DIYbio] Re: Biohacking Start Equipment
The-Odin.com has everything you need at really cheap prices.Thanks for all the Answers.--I currently try to build my on PCR. The Question is now:
Which Primer and Polymeras do i need? Where can i buy the chemicals?
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Re: [DIYbio] Re: Biohacking Start Equipment
Thanks for all the Answers.--I currently try to build my on PCR. The Question is now:
Which Primer and Polymeras do i need? Where can i buy the chemicals?
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[DIYbio] Re: Biohacking Start Equipment
Which Primer and Polymeras do i need? Where can i buy the chemicals?
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Re: [DIYbio] Biorad micropulser electroporator question
On Saturday, October 29, 2016 at 11:34:58 PM UTC-4, Nathan McCorkle wrote:
You were using an electroporation cuvette, right? (I can't tell)
Here is a protocol that worked well for me, with E.coli overnight
subcultures and 18MOhm water... your medium may have been too salty
(conductive):
https://groups.google.com/forum/#!msg/diybio/ JcKyNIQGLIM/u4qIOllQRnAJ
Also, FYI:
Here are tables of voltage settings for various organisms...
http://m.imgur.com/a/swOXq
Also from this BioRad doc, pg 28
http://www.bio-rad.com/webroot/web/pdf/lsr/ literature/4006174B.pdf
(screenshot here too) http://imgur.com/TmwPOho
Most Interestingly: E.Coli 12-18kV/cm, test at 5msec pulse, adjust
from that starting point for efficiency tuning
S.cerevisieae ~7.5kV/cm, start at 5msec pulse
On Sat, Oct 29, 2016 at 6:59 AM, tom a. <jup...@gmail.com> wrote:
> Does anyone have any experience with a Biorad MicroPulser Electroporator? I
> made sure that the metal prongs were touching the metal sides of the
> cuvette, but it still errored out. I've never used one before, and want to
> make sure I'm doing it right.
>
> Thanks in advance,
> Tom
>
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Re: [DIYbio] Biorad micropulser electroporator question
You were using an electroporation cuvette, right? (I can't tell)
Here is a protocol that worked well for me, with E.coli overnight
subcultures and 18MOhm water... your medium may have been too salty
(conductive):
https://groups.google.com/forum/#!msg/diybio/JcKyNIQGLIM/u4qIOllQRnAJ
Also, FYI:
Here are tables of voltage settings for various organisms...
http://m.imgur.com/a/swOXq
Also from this BioRad doc, pg 28
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006174B.pdf
(screenshot here too) http://imgur.com/TmwPOho
Most Interestingly: E.Coli 12-18kV/cm, test at 5msec pulse, adjust
from that starting point for efficiency tuning
S.cerevisieae ~7.5kV/cm, start at 5msec pulse
On Sat, Oct 29, 2016 at 6:59 AM, tom a. <jupm19@gmail.com> wrote:
> Does anyone have any experience with a Biorad MicroPulser Electroporator? I
> made sure that the metal prongs were touching the metal sides of the
> cuvette, but it still errored out. I've never used one before, and want to
> make sure I'm doing it right.
>
> Thanks in advance,
> Tom
>
> --
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[DIYbio] Meetup in Boston this afternoon
Hi DIYBio folks,
As a number of you may be visiting Boston for iGEM, feel free to drop by our space in Somerville this afternoon (1-5pm). We'd love to meet you and show you our humble space!!
339 Summer St, Somerville, MA 02144
Warmly,
Francis
Boston Open Science LAB (BOSlab)
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[DIYbio] Biorad micropulser electroporator question
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Urgently looking for SAP FICO functional Consultant(Local) || Sunnyvale, CA
Dear Partner,
Hope you are doing great!!
Please go through the requirement and let me know if you are having any consultant for this position.
Please share me the profile asap as this is a very hot requirement.
SAP FICO functional Consultant
Sunnyvale, CA(Local)
6+ Months
CMT Level 9
CONFIGURATION EXPERIENCE IS REQUIRED (will be tested for during interview)
SAP functional experience specializing in design and configuration of SAP Finance modules. Experience in full implementation of SAP FI modules.
Experience in gathering business requirements, providing conceptual and detailed designs to meet business needs, performing necessary SAP configurations, writing detail specifications for development of custom programs, testing, co-ordination of transports to production and post go live support.
Requires excellent verbal and written communication skills, including presentations creation and delivery.
Minimum 5 years of SAP FICO / FI-CA experience. Good understanding of accounting concepts.
Bachelors Degrees and Post graduate degree (MBA / CA) is required.
Prior experience in high tech or software industries is preferred
Start Time:12:00 AM
Hours:8:00am to 5:00pm
Best Regards,
Arpit Arora
Pyramid Consulting, Inc.
Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home
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Urgently looking for Support Engineer(Local) || Sunnyvale, CA
Dear Partner,
Hope you are doing great!!
Please go through the requirement and let me know if you are having any consultant for this position.
Please share me the profile asap as this is a very hot requirement.
Sunnyvale, CA
6+ Months
Extensive experience in providing L1 & L2 support for enterprise systems
Well verse with the use of XML, JSON and REST services
Working knowledge of UNIX, MAC OS X, iOS, is a plus
Solid understanding of data modeling and database systems including Oracle, mySQL, NOSQL, etc.
Solid knowledge of applications performance improvement techniques and caching solutions
Working knowledge of source control software (SVN or Git).
Experience with build and deployment tools, e.g., Maven, Gradle.
Ability to multi-task and manage tasks with varying priorities.
Ability to work independently with minimal supervision
Excellent written and oral communication skills
Responsibilities:
Provide L1 & L2 support for multiple home grown services and frameworks (Platform Services)
Work with application teams to provide guidance and assist them with onboarding process
Develop custom utilities needed by application teams in order to onboard to platform services
Build and enhance standard operating procedures for all platform services and frameworks
Standardize deployment & onboarding playbooks for all supported services
Support deployment of applications and services - assist DevOps engineers
Take over SRE responsibilities for all hosted platform services
Start Time:12:00 AM
Hours:8:00am to 5:00pm
Best Regards,
Arpit Arora
Pyramid Consulting, Inc.
Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home
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Urgently looking for SAP FICO functional Consultant(Local) || Sunnyvale, CA
Dear Partner,
Hope you are doing great!!
Please go through the requirement and let me know if you are having any consultant for this position.
Please share me the profile asap as this is a very hot requirement.
SAP FICO functional Consultant
Sunnyvale, CA (Local)
6+ Months
CMT Level 9
CONFIGURATION EXPERIENCE IS REQUIRED (will be tested for during interview)
SAP functional experience specializing in design and configuration of SAP Finance modules. Experience in full implementation of SAP FI modules.
Experience in gathering business requirements, providing conceptual and detailed designs to meet business needs, performing necessary SAP configurations, writing detail specifications for development of custom programs, testing, co-ordination of transports to production and post go live support.
Requires excellent verbal and written communication skills, including presentations creation and delivery.
Minimum 5 years of SAP FICO / FI-CA experience. Good understanding of accounting concepts.
Bachelors Degrees and Post graduate degree (MBA / CA) is required.
Prior experience in high tech or software industries is preferred
Start Time:12:00 AM
Hours:8:00am to 5:00pm
Best Regards,
Arpit Arora
Pyramid Consulting, Inc.
Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home
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[DIYbio] Re: Using Crispr on plants
On Wednesday, October 26, 2016 at 5:03:47 PM UTC-7, Anonymous Anonymous wrote:
Hello, I'm interested in getting started in DIYbio, I live in Miami where there doesn't really seem to be a community for DIYbio.I think I could scrape some money together for basic lab equipment, but my main question is, what is the primary procedure for using Crispr in plants?From the articles I've read, it seems that the only eukaryotic organism that's easily "Crispr'd" is yeast, although labs seem to be moving to plants.Is using Crispr to gene-edit plants within the reach of an amateur?
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Re: [DIYbio] Using Crispr on plants
Sebastian S. Cocioba
--
Hello, I'm interested in getting started in DIYbio, I live in Miami where there doesn't really seem to be a community for DIYbio.I think I could scrape some money together for basic lab equipment, but my main question is, what is the primary procedure for using Crispr in plants?From the articles I've read, it seems that the only eukaryotic organism that's easily "Crispr'd" is yeast, although labs seem to be moving to plants.Is using Crispr to gene-edit plants within the reach of an amateur?
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Required || Devops Engineer || Sunnyvale, CA(Local)
Dear Partner,
Hope you are doing great!!
Please go through the requirement and let me know if you are having any consultant for this position.
Please share me the profile asap as this is a very hot requirement.
Devops Engineer
Sunnyvale, CA
Need Local
6+ Months
Minimum 5-7 years experience of application build and release engineering in a large scale environment including:
• Developing Puppet modules to automate application installation and configuration management
• Administering Oracle Enterprise Linux including Yum and RPM packaging
• Experience installing, integrating and running Docker containers
• Configuring the Jenkins continuous integration server
• Automating Java application builds using tools such as Ant and Maven
• Using Git and Gerrit distributed source code control
• Experience installing, integrating and running Docker containers
• Splunk power user and ability to create dashboard
Best Regards,
Arpit Arora
Pyramid Consulting, Inc.
Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home
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[DIYbio] Using Crispr on plants
Hello, I'm interested in getting started in DIYbio, I live in Miami where there doesn't really seem to be a community for DIYbio.
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Urgent need of Hyperion Related multiple roles in in Sunnyvale, CA
Dear Partner,
Hope you are doing great!!
Please go through the requirement and let me know if you are having any consultant for this position.
Please share me the profile asap as this is a very hot requirement.
Hyperion Consultant
Location: Sunnyvale, CA
Duration:6+ months
Job Description:
1. ETL JD -Must Have
· 8+ years of experience working in an environment with FDM and HFM
· Experience with FDM 11.1.2.3+ version upgrades and/or development
· Strong experience with Python or VB script
· Strong business rules skills in FDM mapping and data load rules
· Excellent knowledge on EBS ( definitely GL)
· Strong Integration and automation experience with FDMEE or other ETL tools
· Strong knowledge of other ETL tool options
· Strong functional knowledge of Consolidation processes.
· Strong programming and problem solving skills with PL/SQL
· Strong oral and written communication skills
· Attention to details is critical for this role.
· Excellent problem solving skills
Preferred
· Accounting background or strong familiarity with accounting and close processes
· Ability to interact with business and technical teams
Title: EPM (HFM/DRM) Senior Analyst
Location: Sunnyvale, CA
Duration:6+months
Job Description:
Must Have 7+ years of experience working in an environment with HFM 7+ years of Accounting background, in particular strong practical (hands on) experience with Accounting Close and Consolidation processes;
· 7+ years of experience with data integration between multiple systems;
· 2+ years of master data management; Strong Functional knowledge of HFM business rules, EPMA, FDM;
· Strong knowledge of FR Reporting (including build);
· Exceptional problem solving and data reconciliation skills (including data and metadata)
· Strong oral and written communication skills Ability to interact with business and technical teams Attention to details is critical for this role.
· Ability to work independently;
· Excellent organizational and multi-tasking skills
Preferred Experience
· HFM 11.1.2.4 version;
· Experience with Oracle GL;
· Experience with Data Relationship Management (DRM) concepts and/or Chart of Accounts redesign or mapping initiatives;
· Experience with COA approval workflows and master data governance
3.HFM Developer
8+ years of experience working in an environment with HFM and FDM • Experience with HFM 11.1.2.4 version upgrades and/or development • Strong business rules skills in HFM • Strong Integration and automation experience with FDMEE • Strong functional knowledge of Consolidation processes. • Strong programming and problem solving skills with PL/SQL • Strong oral and written communication skills • Attention to details is critical for this role. • Excellent problem solving skills Preferred • Accounting background or strong familiarity with accounting and close processes • Ability to interact with business and technical teams
4.HFM Analyst
Must Have 7+ years of experience working in an environment with HFM 7+ years of Accounting background, in particular strong practical (hands on) experience with Accounting Close and Consolidation processes 7+ years of experience with data integration between multiple systems Strong Functional knowledge of HFM business rules, EPMA, FDM Strong knowledge of FR Reporting (including build) Exceptional problem solving and data reconciliation skills (including data and metadata) Strong oral and written communication skills Ability to interact with business and technical teams Attention to details is critical for this role. Ability to work independently Previous experience with HFM upgrade Excellent organizational and multi-tasking skills Preferred Experience with HFM 11.1.2.4 version upgrades and/or development Experience with Oracle GL Experience with FDM EE 11.1.2.3
Best Regards,
Arpit Arora
Pyramid Consulting, Inc.
Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/
We Find Hidden Talent
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