Thanks for the tips. I am using an electroporation cuvette. I tried it without a cuvette, and got the same result. I did notice that the slider thing was upside down, but even after correcting that, it had the same result of Error.
On Saturday, October 29, 2016 at 11:34:58 PM UTC-4, Nathan McCorkle wrote:
-- I actually tried using DI water, but had the same error. I think I might take it apart and see if the prongs on the sides are loose.
Thanks again for your help.
--Tom
On Saturday, October 29, 2016 at 11:34:58 PM UTC-4, Nathan McCorkle wrote:
You were using an electroporation cuvette, right? (I can't tell)
Here is a protocol that worked well for me, with E.coli overnight
subcultures and 18MOhm water... your medium may have been too salty
(conductive):
https://groups.google.com/forum/#!msg/diybio/ JcKyNIQGLIM/u4qIOllQRnAJ
Also, FYI:
Here are tables of voltage settings for various organisms...
http://m.imgur.com/a/swOXq
Also from this BioRad doc, pg 28
http://www.bio-rad.com/webroot/web/pdf/lsr/ literature/4006174B.pdf
(screenshot here too) http://imgur.com/TmwPOho
Most Interestingly: E.Coli 12-18kV/cm, test at 5msec pulse, adjust
from that starting point for efficiency tuning
S.cerevisieae ~7.5kV/cm, start at 5msec pulse
On Sat, Oct 29, 2016 at 6:59 AM, tom a. <jup...@gmail.com> wrote:
> Does anyone have any experience with a Biorad MicroPulser Electroporator? I
> made sure that the metal prongs were touching the metal sides of the
> cuvette, but it still errored out. I've never used one before, and want to
> make sure I'm doing it right.
>
> Thanks in advance,
> Tom
>
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