Thanks Andreas for your input,
On Saturday, 1 October 2016 19:42:55 UTC+2, Mega [Andreas Stuermer] wrote:
-- I agree, a lot of what we know we learn by doing, also through interacting with others on forums such as this one, and in formal training as well.
But remember I'm a mechanical engineer with high school and college level on biology and chemistry. I've always been interested in science in general including genetics, but i don't have the basics. I like to understand what i'm doing, how things work, so i can combine ideas and eventually find something novel or interesting.
2 weeks ago i would have read your first phrase below about enzymes and amino acids and i would not know what these are. Now i'm at the end of course 3 (out of 10) of Edx MITx: 7.00x Introduction to Biology , and because of this i better understand what you're describing.
But the rest of your procedure is still fuzzy. I do hope when i finished this first MITx course (and the 2 others proposed in this thread), i'll be able to make simple experiments on my own, with the help of forums members and build upon that.
I don't have yet a specific project in mind, but i'm pretty good at putting odd things together and coming up with interesting stuff sometimes. I believe coming from an unrelated field has disadvantages but it also makes it easier to think outside the box and explore paths that veterans would not bother to walk because of their assumptions.
thanks again for your input,
On Saturday, 1 October 2016 19:42:55 UTC+2, Mega [Andreas Stuermer] wrote:
I found that learning by doing is most effective. Do you have a project in mind?E.g., immagine you want to genetically engineer strawberries that taste like banana.There http://openwetware.org/wiki/IGEM:MIT/2006/Blurb is the circuit.You need these 3 enzymes that convert the amino acid leucine into isoamyl acetate (aka banana smell compound)
- <bbpart>BBa_J45008</bbpart>: BAT2 - enzyme that catalyzes the first step in isoamyl alcohol biosynthesis from L-leucine (endogenous amino acid)
- <bbpart>BBa_J45009</bbpart>: THI3 - enzyme that catalyzes the second step in isoamyl alcohol biosynthesis
- <bbpart>BBa_J45014</bbpart>: ATF1 - enzyme that converts isoamyl alcohol to isoamyl acetate
Then you google plant expression vectors and will find pCambia.Then you add the genes for the three proteins into the plant vector, put it into agrobacterium and transform strawberries.On the way you will learn the techniques - miniprep. restriction digest, ligation, etc.Unless you need letters to put after your name like BSc, MSc, PhD. Unnecessary (but good) if you do a start-up yourself.Andreas
On Saturday, September 24, 2016 at 10:04:09 PM UTC+2, Hugues wrote:Hello DIYBio !I'm looking for one of the best and up to date online course on genetics engineering, as an introduction and to get the basics.I'm a 51 years old engineer currently working in manufacturing in the food industry.I'm preparing a second career and would like to do R&D work initially and potentially start a small bio tech company in the future.I'm looking for an extensive course, free or not, with proper exams and certification, that could be done part time over 1 year or more, and possible advanced courses afterwards.Thanks for your suggestions, so far i have found these 2:I have biology and chemistry level from high school and college, and good scientific background with a growing interest in bio technologies.
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