November 2016 | Science

[DIYbio] Re: How to create a yeast extract from bread making yeast for medium?

2:44 PM |

Hard to say, but if you autoclave it, it should last for several weeks. As far as you keep it sterile should be good enough for your purposes. Keeping it in the fridge will help. Expect some debris on the bottom of your bottle if you store it, even if you filter it (I've seen it even in autoclaved 0.2um-filtered commercial yeast extract).

Good luck!

On Thursday, 1 December 2016 01:19:22 UTC+11, Aubrey wrote:

Do you know how long the extract will last with this?

On Wednesday, November 2, 2016 at 12:19:25 AM UTC-6, Scott wrote:
Hi Aubrey,

Interesting silk project. For your question have a look at this abstract.
https://www.ncbi.nlm.nih.gov/pubmed/20819369

When making media we normally autoclave (~120oC@~20minutes) the yeast extract (and the other components). Try boiling your media for awhile if you don't have a pressure cooker.

Let us know how your project goes.

Cheers,
Scott
http://www.opensciencenet.org

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Urgent Required || Full Stack Java Developer with IVR/Avaya experience || San Jose, CA ||

8:44 AM |

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Full Stack Java Developer with IVR/Avaya Experience

San Jose, CA

6+ Months

Key Skills:

Java, J2EE, Angular, Full Stack

Preferred Qualifications:

Experience with one of the following technologies: JSON, RESTful APIs, App Engine, XML, UML Participation in several full system implementation life-cycles (analyze, design, build, test, implement, support). Solid understanding of information management, data modeling, state machines, system integration, development methodologies (including unit testing) and web technologies. Experience developing call flows Programming experience in an object-oriented language such as Java. Scripting experience with JavaScript. Experience developing business applications including front-end, data storage and application integration. Experience implementing, configuring, customizing and integrating 3rd-party software solutions Experience working on Linux.

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home

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[DIYbio] Re: How to create a yeast extract from bread making yeast for medium?

6:19 AM |

Do you know how long the extract will last with this?

On Wednesday, November 2, 2016 at 12:19:25 AM UTC-6, Scott wrote:

Hi Aubrey,

Interesting silk project. For your question have a look at this abstract.
https://www.ncbi.nlm.nih.gov/pubmed/20819369

When making media we normally autoclave (~120oC@~20minutes) the yeast extract (and the other components). Try boiling your media for awhile if you don't have a pressure cooker.

Let us know how your project goes.

Cheers,
Scott
http://www.opensciencenet.org

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Urgent Required || Full Stack Java Developer || Irvine, CA || Local Only

3:44 PM |

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Full Stack Java Developer

Irvine, CA

6+ Months

F2F (In-Person) Interview

Application Engineer in a team responsible for lead generation efforts at Google as part of the Ads Optimization group. Developed and maintained an internal CRM that applies heuristic filters and machine intelligence to Google's index of 1 billion website in order to find prospects promising in terms of ad spend potential, conversion propensity, seasonal activity & other factors.

* Good knowledge of Core Java
* Good knowledge of Java J2EE architecture
* Good understanding of OOP & Design patterns
* Intermediate knowledge on JavaScript / HTML / CSS
* Good to have SQL knowledge & Application DAO layer

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home

We Find Hidden Talent

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Urgent Required || Full Stack Java Developer || Irvine, CA || Local Only

12:57 PM |

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Full Stack Java Developer

Sunnyvale/ San Jose, CA

6+ Months

F2F (In-Person) Interview

Application Engineer in a team responsible for lead generation efforts at Google as part of the Ads Optimization group. Developed and maintained an internal CRM that applies heuristic filters and machine intelligence to Google's index of 1 billion website in order to find prospects promising in terms of ad spend potential, conversion propensity, seasonal activity & other factors.

* Good knowledge of Core Java
* Good knowledge of Java J2EE architecture
* Good understanding of OOP & Design patterns
* Intermediate knowledge on JavaScript / HTML / CSS
* Good to have SQL knowledge & Application DAO layer

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home

We Find Hidden Talent

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Re: [DIYbio] Plant Tissue Culture first attempt Dallas DIY Bio

9:52 AM |

Its a good idea to replate as soon as the "blood" pools for optimal culturing. It acts as a visual aid to maintain peak media efficiency. Media isnt necessarily for convenience's sake since every orchid species requires slightly more or less extra ingredients (coconut endosperm, banana powder, kinetin, etc.) and its cheaper to just buy the activated charcoal in bulk and have the media blank. Phytotechnologies Laboratories is your friend :)

Sebastian S. Cocioba

CEO & Founder

New York Botanics, LLC

Blog: ATinyGreenCell.com

On Nov 26, 2016, at 6:48 PM, Sebastian S Cocioba <scocioba@gmail.com> wrote:

For orchids best is internodal segments from floral spikes. Ideally from a freshly grown spike that has yet to flower. Leaf tissue is a pain to regenerate from. Aaron Hicks has an orchid seed repository unlike any other. Surface sterilizing seeds is also hit or miss. Best is to hand pollinate a phaleonopsis orchid and take the immature seed pod before it ripens, surface sterilize the pod, and sow the seeds that are intrinsically sterile since the seeds develop from the inside out. Prepare for long haul cultures when it comes to orchids. Set it and forget it. :)

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com

On Nov 26, 2016, at 4:54 PM, Josh Melnick <j.r.melnick@gmail.com> wrote:

Thanks for your help, this was very helpful, I already ordered Tobacco seeds. I was looking at some Orchid Flask videos and yes indeed they have the black agar, from charcoal in it.

On Friday, November 25, 2016 at 8:03:22 PM UTC-6, Sebastian wrote:
Welcome to the wonderful world of plant tissue culture!!!! ::confetti::

Orchids bleed and their blood is toxic to them. Use activated charcoal in orchid media.

Don't start with random plants. Start with tobacco seeds and surface sterilize them. Sprout them and use the resulting aseptic seeds as leaf tissue stock for subsequent practice cultures.

10% household bleach, drop of dish soap, and pre-wash the plant tissue with soap and water manually to get macro-particles off. Soak in bleach for 10-30mins depending on surface area and convoluted tissue structures. Rinse 3x in sterile distilled water. Buy a heap of 6" tweezers and use one tweezer per culture vessel to not spread contam culture to culture.

Orchid media works best with coconut water, a banana, and sucrose. Phytotechlab.com sells everything you need. Plants From Test Tubes 4th edition was my bible when I started. Keep the thread alive and I'll chime in. Best of luck!!!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com

On Nov 25, 2016, at 3:06 AM, Josh Melnick <j.r.m...@gmail.com> wrote:

We are working on building a space here in Dallas, so far our first attempt at Tissue Culture has been rather poor.
About 65% of the vials have mold or fungis, we have quite a good autoclave here, so I will have to work and be much more careful in the future about contamination when putting the plants in the tubes.

I put orchid and rose in the tubes, using multi solution from MicroClone. What is interesting is this black coloration that is coming from the orchids, I suppose this is some product of the plant's metabolism as it is not coming into the other rose plants. I probably should start a blog.

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Urgently looking for a Oracle (PL/SQL) Developer with E-Business and Security in Sunnyvale, CA

8:43 AM |

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Job Title: PL/SQL Developer E-Business and Security experience

Location: Sunnyvale, CA

Duration: 8+ months Contract (Strong chances of extending)

Job Description:

· PL/SQL

· Unix

· Oracle Security(SQL injection)

Skills:

· PL/SQL: Not just concepts. Should have strong experience in writing queries(Mandatory)

· Oracle EBS

· Linux/Unix script

· Resource must have at least around 8 Years of Experience.

· Need to have Skills on Oracle SQL, advance PL/SQL

· Resource must have a very good communication and interpersonal skills

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home

We Find Hidden Talent

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[DIYbio] Re: UV LED for disinfection of water

7:26 AM |

Hey Markos,

I work for AquiSense and got a notification for this thread. We could have a conversation about efficiency of our reactor design and the LEDs we use. Keep in mind that our parent company NIkkiso, manufactures UVC LEDs and we regularly have conversations with customers about using our design or just buying a few LEDs and trying to figure out a design for their own needs. Feel free to contact us and our application engineers can talk specifics on efficiency and price of the project you're looking for. info@aquisense.com

On Thursday, November 24, 2016 at 9:15:38 AM UTC-5, Markos wrote:

Does anyone have experience with commercial products that uses UV LED
for disinfection of water for human consumption?

I found these products but I have no references about efficiency:

http://www.s-et.com/water-disinfection.pdf

http://www.aquisense.com/water-treatment

Any tip?

Thanks,
Markos

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[DIYbio] Re: UV LED for disinfection of water

8:53 PM |

I used to be really into reef aquariums and now I work in municipal water. UV disinfection for aquariums and ponds have been around for around 25 years and basically consist of a UV bulb in a quartz sleeve and water being pumped into the chamber, usually in a coil fashion to maximize contact time. The industrial size treatment we use for the city are essentially the same principal, but on a ginormous scale. There are also built in wipers to clean the quartz sleeve, as any buildup that obstructs the UV will essentially inactivate the disinfection.

I currently use a UV-C CFL from Amazon to disinfect materials that can't be autoclaved. As Patrick said, it can be very dangerous. UVC + O2 = Ozone which can also be hazardous. I have some visible UV LEDs I used for another project that work well, but I haven't seen UVC LEDs for sale yet. What is your final goal? Maybe we can help with some additional ideas.

On Monday, November 28, 2016 at 11:10:50 AM UTC-8, Patrik D'haeseleer wrote:

UVC disinfection for water sterilization has been around for quite a while. In fact, you can buy anything from industrial-scale UV water sterilizers, all the way down to portable "Steripens" for backpackers.

As you discovered, one issue is how low efficiency the UVC bulbs are, which means you pay a lot in electricity cost, and you have to dissipate a lot of heat. Another important limitation is that you need to expose the bacteria to a sufficiently high dose over time to be effective. So it's much easier to expose a bottle of drinking water to constant UV light for a minute, like the Steripen does, than to slap a big LED on the side of your water mains and expect it to be effective.

If you are going to tinker with mercury vapor lamps yourself, BE VERY CAREFUL! Shortwave UVC light is as good as absent from natural sunlight, and can burn out your retinas in a matter of seconds - especially if you work with a strong point source like the 125W bulb in the link that you posted!

(For the same reason, NEVER work at a biosafety cabinet with the sash raised while the UVC germicidal light is on! The glass sash will block most of the UVC, but you can still get a bad sunburn on your arms, or develop the equivalent of snow blindness from the UVC reflected off the stainless surfaces.)

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Re: [DIYbio] Plant Tissue Culture first attempt Dallas DIY Bio

8:15 PM |

I've also just started experimenting with TC and want to try some orchid nodes. One question, it seems like from my reading that charcoal is very much needed. Then why do most vendors sell orchid media without charcoal as an option? Is it marketed towards those who are doing their own experiments or constantly replating?

On Saturday, November 26, 2016 at 3:48:26 PM UTC-8, Sebastian wrote:

For orchids best is internodal segments from floral spikes. Ideally from a freshly grown spike that has yet to flower. Leaf tissue is a pain to regenerate from. Aaron Hicks has an orchid seed repository unlike any other. Surface sterilizing seeds is also hit or miss. Best is to hand pollinate a phaleonopsis orchid and take the immature seed pod before it ripens, surface sterilize the pod, and sow the seeds that are intrinsically sterile since the seeds develop from the inside out. Prepare for long haul cultures when it comes to orchids. Set it and forget it. :)

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com

On Nov 26, 2016, at 4:54 PM, Josh Melnick <j.r.m...@gmail.com> wrote:

Thanks for your help, this was very helpful, I already ordered Tobacco seeds. I was looking at some Orchid Flask videos and yes indeed they have the black agar, from charcoal in it.

On Friday, November 25, 2016 at 8:03:22 PM UTC-6, Sebastian wrote:
Welcome to the wonderful world of plant tissue culture!!!! ::confetti::

Orchids bleed and their blood is toxic to them. Use activated charcoal in orchid media.

Don't start with random plants. Start with tobacco seeds and surface sterilize them. Sprout them and use the resulting aseptic seeds as leaf tissue stock for subsequent practice cultures.

10% household bleach, drop of dish soap, and pre-wash the plant tissue with soap and water manually to get macro-particles off. Soak in bleach for 10-30mins depending on surface area and convoluted tissue structures. Rinse 3x in sterile distilled water. Buy a heap of 6" tweezers and use one tweezer per culture vessel to not spread contam culture to culture.

Orchid media works best with coconut water, a banana, and sucrose. Phytotechlab.com sells everything you need. Plants From Test Tubes 4th edition was my bible when I started. Keep the thread alive and I'll chime in. Best of luck!!!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Blog: ATinyGreenCell.com

On Nov 25, 2016, at 3:06 AM, Josh Melnick <j.r.m...@gmail.com> wrote:

We are working on building a space here in Dallas, so far our first attempt at Tissue Culture has been rather poor.
About 65% of the vials have mold or fungis, we have quite a good autoclave here, so I will have to work and be much more careful in the future about contamination when putting the plants in the tubes.

I put orchid and rose in the tubes, using multi solution from MicroClone. What is interesting is this black coloration that is coming from the orchids, I suppose this is some product of the plant's metabolism as it is not coming into the other rose plants. I probably should start a blog.

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Re: [DIYbio] Questions from a Student

5:44 PM |

I second the kits idea. Carolina, Edvotek and the fine products at The ODIN are all good starters. See if you can expand on the kits or at least post some ideas of what you'd like to do. I'm sure one of us will chime in with some guidance. We wish you all the luck and adventure!

Sebastian S. Cocioba

CEO & Founder

New York Botanics, LLC

Blog: ATinyGreenCell.com

On Nov 28, 2016, at 4:59 PM, Nathan McCorkle <nmz787@gmail.com> wrote:

I would get one of these kits shipped to your school:
http://www.carolina.com/transformation-dna-transfer-kits/ap-biology-lab-6-molecular-biology-module-1-transformation-teacher-demonstration-kit-with-prepaid-coupon/211080.pr

or fashion a similar kit from the various offerings at this
more-friendly store, which offers an even-more entry-level kit as
well:
http://www.the-odin.com/grow-bioluminescent-e-coli-kit/

On Mon, Nov 28, 2016 at 9:57 AM, Ha Anh Le <squiggles15@gmail.com> wrote:
Hi everyone!

My name is Ha Anh and I am a student at Michigan State University. I have a
couple of questions I'm hoping you can answer.

I recently heard about biohacking through a course I am taking. In the same
course, I have decided to do my final project on researching the
accessibility of biohacking and other technologies.

That being said, in the spirit of DIY bio, would anyone have any suggestions
on projects I can do at home without investing in too much equipment? I
would like to do a demo for my class presentation if possible. I've seen a
few really amazing projects but they are difficult to do on a student budget
and a short deadline. I'd really like to be able to use what I have and not
spend too much. Do you have any recommendations?

Also, this one is a bit of a stretch, but would anyone be willing to sit
down for a Skype interview with me? It would be about an hour, and it would
help me out a lot! It would aide in research information for this project.
(I would ask questions like: How did you get involved in diy bio? How long
have you been at it? What projects have you done? etc etc.)

Thank you for taking the time to read this!

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Re: [DIYbio] Questions from a Student

1:59 PM |

I would get one of these kits shipped to your school:
http://www.carolina.com/transformation-dna-transfer-kits/ap-biology-lab-6-molecular-biology-module-1-transformation-teacher-demonstration-kit-with-prepaid-coupon/211080.pr

or fashion a similar kit from the various offerings at this
more-friendly store, which offers an even-more entry-level kit as
well:
http://www.the-odin.com/grow-bioluminescent-e-coli-kit/

On Mon, Nov 28, 2016 at 9:57 AM, Ha Anh Le <squiggles15@gmail.com> wrote:
> Hi everyone!
>
> My name is Ha Anh and I am a student at Michigan State University. I have a
> couple of questions I'm hoping you can answer.
>
> I recently heard about biohacking through a course I am taking. In the same
> course, I have decided to do my final project on researching the
> accessibility of biohacking and other technologies.
>
> That being said, in the spirit of DIY bio, would anyone have any suggestions
> on projects I can do at home without investing in too much equipment? I
> would like to do a demo for my class presentation if possible. I've seen a
> few really amazing projects but they are difficult to do on a student budget
> and a short deadline. I'd really like to be able to use what I have and not
> spend too much. Do you have any recommendations?
>
> Also, this one is a bit of a stretch, but would anyone be willing to sit
> down for a Skype interview with me? It would be about an hour, and it would
> help me out a lot! It would aide in research information for this project.
> (I would ask questions like: How did you get involved in diy bio? How long
> have you been at it? What projects have you done? etc etc.)
>
> Thank you for taking the time to read this!
>
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> Learn more at www.diybio.org
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Required || Java Full Stack Developer || NYC, NY ||

11:30 AM |

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Java Full Stack Developer with Python and NOSQL experience

New York City, NY

6+ Months

Need strong Java developers with NewSQL / NoSQL back-end integration experience to migrate internal applications, batch jobs that use one storage back-end to another. RPC/API style development –Dependency injection. Back end development only – Migrate data using mapped API's – provide migrations for clients needing migration assistance on various platforms.

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com | Linked in: https://www.linkedin.com/in/arpit-arora-55793655 | Web: http://www.pyramidci.com/staffing-home

We Find Hidden Talent

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[DIYbio] Re: UV LED for disinfection of water

11:10 AM |

UVC disinfection for water sterilization has been around for quite a while. In fact, you can buy anything from industrial-scale UV water sterilizers, all the way down to portable "Steripens" for backpackers.

As you discovered, one issue is how low efficiency the UVC bulbs are, which means you pay a lot in electricity cost, and you have to dissipate a lot of heat. Another important limitation is that you need to expose the bacteria to a sufficiently high dose over time to be effective. So it's much easier to expose a bottle of drinking water to constant UV light for a minute, like the Steripen does, than to slap a big LED on the side of your water mains and expect it to be effective.

If you are going to tinker with mercury vapor lamps yourself, BE VERY CAREFUL! Shortwave UVC light is as good as absent from natural sunlight, and can burn out your retinas in a matter of seconds - especially if you work with a strong point source like the 125W bulb in the link that you posted!

(For the same reason, NEVER work at a biosafety cabinet with the sash raised while the UVC germicidal light is on! The glass sash will block most of the UVC, but you can still get a bad sunburn on your arms, or develop the equivalent of snow blindness from the UVC reflected off the stainless surfaces.)

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Re: [DIYbio] Re: UV LED for disinfection of water

10:00 AM |

Hi Markos
Check amazon etc for fluorescent UVC germicidal lamps. They cost in the $10's range for 20 to 40W. You would still need the fluorescent drivers, either ballasts from regular fluorescent tubes or the electronics from CFLS.
Good luck
Abizar

On Nov 28, 2016 4:35 AM, "Markos" <markos@c2o.pro.br> wrote:

Hi Patrik,

I tried to know the prices in these 2 companies but until now I have had no answer.

As a cheaper alternative, I'm thinking about using the mercury vapor lamp that are used inside the bulbs used in street lighting.

As described at:
http://www.pcbheaven.com/projectpages/Hack_Hg_Lamp_to_UV_Light_/

But I think I'll have to use some system to cool the lamp which, depending on the power, dissipate too much heat.

My idea is to keep that lamp, in horizontal position, above a water reservoir with a small depth to allow UV penetration to the bottom of the reservoir.

That way I would not need to use a quartz tube to insulate the bulb from the water.

What do you think?

Thanks,
Markos

Em 25-11-2016 06:22, Patrik D'haeseleer escreveu:

Seems legit. Do keep in mind that you need very short-wave UV C light to kill bacteria, and LEDs get exorbitantly expensive in those wavelengths. These are definitely not your typical 10 cent near-UV LEDs. These could easily cost a couple hundred dollars per LED.

Patrik

On Thursday, November 24, 2016 at 6:15:38 AM UTC-8, Markos wrote:
Does anyone have experience with commercial products that uses UV LED
for disinfection of water for human consumption?

I found these products but I have no references about efficiency:

http://www.s-et.com/water-disinfection.pdf

http://www.aquisense.com/water-treatment

Any tip?

Thanks,
Markos

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[DIYbio] Questions from a Student

9:57 AM |

Hi everyone!

My name is Ha Anh and I am a student at Michigan State University. I have a couple of questions I'm hoping you can answer.

I recently heard about biohacking through a course I am taking. In the same course, I have decided to do my final project on researching the accessibility of biohacking and other technologies.

That being said, in the spirit of DIY bio, would anyone have any suggestions on projects I can do at home without investing in too much equipment? I would like to do a demo for my class presentation if possible. I've seen a few really amazing projects but they are difficult to do on a student budget and a short deadline. I'd really like to be able to use what I have and not spend too much. Do you have any recommendations?

Also, this one is a bit of a stretch, but would anyone be willing to sit down for a Skype interview with me? It would be about an hour, and it would help me out a lot! It would aide in research information for this project. (I would ask questions like: How did you get involved in diy bio? How long have you been at it? What projects have you done? etc etc.)

Thank you for taking the time to read this!

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[DIYbio] Re: UV LED for disinfection of water

2:37 AM |

Hi Patrik,

I tried to know the prices in these 2 companies but until now I have had no answer.

As a cheaper alternative, I'm thinking about using the mercury vapor lamp that are used inside the bulbs used in street lighting.

As described at:
http://www.pcbheaven.com/projectpages/Hack_Hg_Lamp_to_UV_Light_/

But I think I'll have to use some system to cool the lamp which, depending on the power, dissipate too much heat.

My idea is to keep that lamp, in horizontal position, above a water reservoir with a small depth to allow UV penetration to the bottom of the reservoir.

That way I would not need to use a quartz tube to insulate the bulb from the water.

What do you think?

Thanks,
Markos

Em 25-11-2016 06:22, Patrik D'haeseleer escreveu:

Seems legit. Do keep in mind that you need very short-wave UV C light to kill bacteria, and LEDs get exorbitantly expensive in those wavelengths. These are definitely not your typical 10 cent near-UV LEDs. These could easily cost a couple hundred dollars per LED.

Patrik

On Thursday, November 24, 2016 at 6:15:38 AM UTC-8, Markos wrote:
Does anyone have experience with commercial products that uses UV LED
for disinfection of water for human consumption?

I found these products but I have no references about efficiency:

http://www.s-et.com/water-disinfection.pdf

http://www.aquisense.com/water-treatment

Any tip?

Thanks,
Markos

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[DIYbio] Publication on simple DIY real-time PCR system

1:02 AM |

this was a fascinating read. PCR with a constant-on heater, real time PCR with a phone camera plus a quadracoptor thrown in for good measure!

Truly great ideas in this publication.

http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b04153

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Re: [DIYbio] Re: Extracting substances from a chromotography paper

10:44 PM |

Hi Aubrey, you need 100s mg/L for tissue culture. Hair hydrolysis also requires a lot more effort. Egg white might be a better starting point. TLC wont produce enough materials though, you might have to go to column chromatography but then buying amino acids turns out to be much cheaper. Mammalian tissue culture media is not that expensive, 500 ml costs about $30.

On Sat, Nov 26, 2016 at 9:03 PM, Koeng <koeng101@gmail.com> wrote:

Yes, but you'd likely get trace quantities that aren't all that useful. What is the goal?

On Saturday, November 26, 2016 at 12:01:57 PM UTC-8, Aubrey wrote:
So if I were to extract amino acids by hydrolyzing human hair, and then separated them out with chromotography paper, then identified each one according to reference Rf values, could I then be able to then remove the amino acids from the paper and convert them to their powdered form?

I had an idea that if I cut up the paper (separating each type of amino acids) and then dunk it in pure water the amino acids dissolve into the water and I can then remove the paper and allow the water to evaporate leaving the amino acids behind.

Thoughts?

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