[DIYbio] How to create a yeast extract from bread making yeast for medium?

As far as I know, yeast extract is made by cooking the yeast at 55 degrees Celsius roughly. At this temperature its own enzymes such as protease and lipases degrade the cells (Autolysis). Probably afterwards it is cooked 65 degrees to kill off any non-spore forming bacteria, or autoclaved

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