Hello DIY Bio Community!
-- My name is Han. I am a student who's been interested in DIY Bio, and I wanted to undergo my own project. I have recently been following the JHU 2011 VitaYeast IGEM project (http://2011.igem.org/Team:Johns_Hopkins) in an attempt to recreate their yeast transformation as a fun way to get started. Electroporation systems have been a little out of my budget for a beginner procedure, so I'm sticking with a lithium acetate transformation instead.
However, I have had a bit of trouble searching for salmon sperm DNA that I can use, and I was wondering if you all had any cheap sources for to find it or ideas for replacement materials? I was also wondering how necessary it is to include in the transformation, and if I can simply continue on with just DMSO instead?
Thank you in advance, and best of luck in your own projects,
Han
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