Re: [DIYbio] Re: My first successful CRISPR experiment on E. Coli ?

On 01/01/2017 01:52 PM, Hugues wrote:
> Following up on my earlier experiment,
>
> I have scraped off "mutant" bacteria from the strep plate and spread it onto a fresh strep plate and a normal plate.
>
> I have scraped off wild type bacteria from the strep plate and spread it onto a fresh strep plate and a normal plate.
>
> The first picture below shows the plates just after a spread the bacteria on them.
>
> The second picture is of the same two plates after 2 days of incubation at around 37 C.

You have spread a thick layer of bacteria, since it is visible just after transferring it. It obviously grew.
Wouldn't it be good to use streaking technique to see the results of some individual cells growing into colonies?
That might show differences between colonies if you are starting with a mixture. What you have here looks like colonies
touching each other.

What does that mean when you say, "I guess there is growth on all 4 colonies"? Is the first picture after
enough time for growth?

The left right growth rates do look close to the same.

"Maybe there is no streptomycin finally on that plate" What is a good simple test of that?

"it controls algae in ornamental ponds and aquaria." I have no experience with this -- just found it on wikipedia, but
it might be an easy test of whether you have streptomycin that is still effective. See if your boiled streptomycin
kills pond algae faster than boiled rain water side by side.

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