Re: [DIYbio] Could we use optical heterodyning to boost UV bands into VIS?

On Wed, Jun 14, 2017 at 1:47 AM, John Ladasky <john.ladasky@gmail.com> wrote:
> I'm not sure exactly what this has to do with biology, but...

Mostly regarding the detection of biomolecules. I was thinking about
how to use cheaper light sources to substitute for less common
wavelengths, i.e. 260 and 280 nm.
The article I just posted is specifically about DNA research, and
implications of surface hydration effects on DNA interactions with
proteins, drugs, etc. It sounds pretty much like two-photon

> The standard 532 nm laser is actually a 1064 nm laser with a
> frequency-doubling crystal. Trying to make this relevant to biology,
> there's a technique called two-photon excitation microscopy

I'm familiar with both those concepts, I guess SFG is just a different
term for detecting the two-photon emissions that are characteristic of
certain molecules, which seem much weaker than the common two-photon
dyes. I wonder if it is a similar order of magnitude difference
between fluorescense and Raman scattering (reading now).

Very cool!

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