The plasma requires an origin of replication that works with the host. Different plasmids tend to be maintained at different number copies per cell, but common bacterial plasmids generally have many copies per cell and will continue to be copied and replicated with the cells (unless there is selective pressure to loose the plasmid).
On Tue, Sep 19, 2017 at 11:49 AM Rikke Rasmussen <rikke.c.rasmussen@gmail.com> wrote:
In bacteria, at least, the plasmids are replicated with the cell cycle - it's how "we" make more plasmid. =)--On Tue, Sep 19, 2017 at 9:33 AM, Cory Geesaman <cory@geesaman.com> wrote:The title pretty much has the substance of this. I'm curious if you can introduce plasmids to a cell via electroporation and have those plasmids replicated in subsequent cell divisions, or if they only end up in one of the cells while multi-generational versions must be incorporated into the DNA somehow. Would the answer differ for different types of cells?--
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