Bacteria are not hamburgers. Much better shelf life. If I put E. coli in a tube (under more or less sterile conditions) there are still be some viable bugs in there after a few months, usually even years… this is my personal experience – but I'm looking forward to be contradicted.
Re-testing a fresh sample makes total sense - if we want to see if the problem was fixed in the meantime. Any volunteer with a sequencer?
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