A concern is the discharge coupling RF energy into nearby electronics which scrambles the microcontroller, resulting in random software behavior. No joke. Any part of the design using solid state electronics (that means anything with a microcontroller especially) should have significant shielding and ground isolation and redundancy. The resulting errors would occur at random and very difficult to reproduce or troubleshoot yet could be dangerous because it would lead to unexpected program operation. An analog electronics control circuit could be better, or at least reduce software's control over the final discharge. Consider how much effort Toyota went through to attempt to debug the deadly Prius stuck-accelerator problem (and their internal investigation was forced by external pressure, several years after Steve Wozniak suggested there was a software bug in his own Prius) and still perhaps Toyota was unable to diagnose a specific semiconductor or software based fault in their design (at least publicly disclosed.. I'm sure their investigation found several unrelated software bugs which passed their original Q-A).
On Tuesday, May 1, 2018 at 10:02:06 PM UTC-7, James Feeney wrote:
-- For example. Rather than the microcontroller and software directly controlling the trigger (entirely RAM-based logic and prone to faults), the microcontroller and software could reset an external timer chip which employs analog components to set specific periodicity for discharge and is design-limited by those components to only discharge after another time-window has elapsed. Etc. Fail-safes with built-in redundant fail-safes.
On Tuesday, May 1, 2018 at 10:02:06 PM UTC-7, James Feeney wrote:
Cory,I have been looking at this. I'm thinking Tigon tubing, mineral oil, and stop leak powered aluminum radiator treatment for the metal particles. A small pump, HV source, and vioula liquid Van De Graaff generator. There's technically no limit to the Voltage except leakage. The better you can control leakage, the higher the Voltage possible. Maybe instead of mineral oil, silicone oil. Then the challenge, where are you going to store those SuperHV charges? Whatever it is, it had better be inside a real good insulator.
On Thursday, April 5, 2012 at 11:23:24 PM UTC-7, Jonathan Cline wrote:
On Monday, March 12, 2012 2:17:12 PM UTC-7, Mega wrote:This http://mosfetkiller.de/?s=kaskaden
describes a cascade.
It's used for doubling, (depending on how much condensators u use...
*2 *4 *8 *16 ....) input voltage....
I posted my quick circuit layout for a voltage ladder (tripler) some time back.
Here's the link to the schematic, layout, and complete parts list for downloading.HVPS for Systems Biology: A Low Cost, High Voltage Power Supply with Schematics + Board Layout
http://88proof.com/synthetic_biology/blog/archives/303
I used a european auto inverter (12VDC->220VAC) to feed mine, which yielded over 900V sustained, 1200V peak. It is useful in certain situations, though there are limitations to this type of circuit. Stay away from the leads when it is operational, and even for many minutes after powering it down (and even after forcibly discharging it).
I believe that MIT has students build these boards in certain EE lab classes.
I think it could be used for electroporation, however the plates would have to be precise gap width, and pulse duration would not be very predictable at all. (Some papers suggest that pulse duration is not critical...)
Enjoy
## Jonathan Cline
## jcl...@ieee.org
## Mobile: +1-805-617-0223
########################
--
## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/90677751-5bc5-4a33-ab5f-0f8ddb430fae%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment