Re: [DIYbio] Opentrons OT-2 release

Antha is also pretty pricey per year, I think 4 times an OT cost?

That said, I met with some developers of Antha and I really like it. Their GUI is really great for management and wonderful for multifactorial optimization. Their data from optimizing GoldenGates was very impressive. It's a piece of proprietary software I would consider purchasing, if I had the money to, since mainly what you're paying for is support and the user interface supporting the bottom-level code. Also, yea it's not really vendor lock-in because it works across many platforms - but python is pretty hard to beat when working with libraries.

Here are some videos of our OT1s doing work with our codebase. When in "build-mode" we run about 3 at a time, cloning about 300 plasmids a day. 

OT doing GoldenGates

https://www.youtube.com/watch?v=-_tEMNG0I3U

OT doing transformations

https://www.youtube.com/watch?v=IQA4v-9h0W8

OT doing plating (from above)

https://www.youtube.com/watch?v=FxHn1avxR-4

OT doing plating (from back)

https://youtu.be/xXuF4v3vZ78?t=11s

(At the end of this video, you can see how we're doing the serial dilutions. Extremely important to note: you want to slowly dispense the liquid above the plate, creating a small droplet that sticks to the sides of the tip, and THEN move down to drop the droplets while stabbing the agar a little. If you do it when you're already down in the agar, you're gonna have a bad time)

Koeng

On Wednesday, June 27, 2018 at 11:30:34 PM UTC-7, Michael Crone wrote:

Antha removes the vendor "lock-in" that you are talking about and allows for cross-platform workflows (which becomes useful when you are working in a DNA foundry environment). And when you already have a $30 000 robot it's difficult to justify to your PI to buy a $10 000 one... especially when that robot is already fully equipped with all of the accessories.

I completely agree though that the opentrons is great for every day work in the lab and its programmability makes it very attractive.

On Wednesday, 27 June 2018 17:02:22 UTC+2, John Griessen wrote:

On 06/27/2018 02:42 AM, Michael Crone wrote:
> We have a Felix in my lab and I'd like to get it up and running because it is easier to justify using that than buying the cheaper opentrons.

What makes it easier to justify?  After Koeng's email on how wonderful programmability without vendor lock-in is, I don't get you.