There are many different variations on the protocol but Wikipedia has a basic overview of the technique. The key is picking enzymes that aren't too rare otherwise your PCR product may be too large. A priori you won't know how far the sites are from the integrant so you may have to try a few different enzymes.
-- https://en.wikipedia.org/wiki/Inverse_polymerase_chain_reaction
HTH,
Scott
On Thursday, July 12, 2018 at 10:26:15 AM UTC-7, Skyler Gordon wrote:
On Thursday, July 12, 2018 at 10:26:15 AM UTC-7, Skyler Gordon wrote:
How can you be sure that you're digesting in the right locations if you don't know the location of your insertion site? It seems like it could provide 90% accurate data, but the chance of losing something due to fragment sizes being too large or small is there.
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