On 10/26/18 7:57 PM, Andreas "Mega" Stuermer wrote:
> First project: creating the empty vector, SciHouse in Florida will store the vector in bulk and share with anyone interested (open
> source)

How do you do transformation?  Is electroporation used?  I'd like more beta testers of my electroporator.
So far, the folks that have gotten one have never used it on live cells.  Kind of slows the project momentum...

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/xS-TUbOqf1o/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/b2c7b8cc-008d-2307-f54d-0b6443c04b64%40industromatic.com.
For more options, visit https://groups.google.com/d/optout.

I have seed from my gleaux plant, a bunch that dropped into the pot
that it was planted in have sprouted so I know they're viable. I never
saw the original glow, nor these... and they don't show up with a 30
second dSLR exposure image. I had been working to setup my laptop to
remotely trigger my camera to take a series of images and then add a
bunch of images together to try stack the brightness... but I got
caught up with other things and then my laptop went through 3 weeks of
failed ubuntu upgrade attempts before I got it straightened out again
recently.

Anyway, if you think you could use some seed, how many would you want?
On Fri, Oct 26, 2018 at 5:57 PM Andreas "Mega" Stuermer
<andreas.t.stuermer@gmail.com> wrote:
>
> Hi guys,
>
> I am currently re-visiting the idea of chloroplast engineering. Chloroplast engineering yields loads of proteins, and is only maternally inherited (containment bonus). Antibiotic selection marker will be removable with Cre-Lox, and should happen with low frequency by iself too.
>
> First project: creating the empty vector, SciHouse in Florida will store the vector in bulk and share with anyone interested (open source)
> Second project: putting the lux operon in, just because. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015461 *
> I have seen one of these plants, and they were visibly glowing to the naked eye (but weak). Note that that was way before the Silicon Valley glowing plant cowboy project.
>
> *Unfortunately they stopped selling them in the United States for no apparent reason (maybe they didnt't get enough customers).
>
> Not sure if I should start with weeping willow or tobacco. I've been routinely working with tobacco and can share my protocols with our US collaborators so they can do the tissue culture there. Tobacco is pretty lame though.
>
> Anyways, just wanted to see if anyone would be willing to chime in, synthesis is expected to cost around 500$. Attached the file. Addition of lux will just be one PCR step
>
>
>
>
> --
> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
> To post to this group, send email to diybio@googlegroups.com.
> Visit this group at https://groups.google.com/group/diybio.
> To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/a90a14a1-7960-415a-976d-2ce034fcaa74%40googlegroups.com.
> For more options, visit https://groups.google.com/d/optout.



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/xS-TUbOqf1o/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9JtQ5eXYE_7sJYsfZ0jVaLMmvLrwPh52dve%3D3K7pgcJhA%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

You're totally spot on here, Cathal. Cookbooks for peanuts. Cookbooks for carbon.

More uses of carbon dioxide would change the game. Right now it's treated as a nearly worthless burden ($40/ton) that civilization has to bury underground or turn away from. The IPCC modeled a tax of up to $27,000 per ton of carbon dioxide by 2100 though!

Our team wanted to explore high-value uses of carbon. We set out to make a diamond using atmospheric carbon dioxide. Turns out that's really hard. So we made a decorative planter out of air instead: http://airminers.org

Tito

On Tuesday, October 2, 2018 at 11:59:32 PM UTC-7, Cathal Garvey wrote:

Thinking-emoji-face.. When the USDA wanted to make peanuts a thing, they created cookbooks of things you could do with peanuts. It worked.

We have some industrial processes already that use supercritical CO2. Perhaps if we devised more uses, especially ones that would be awesome but are uneconomical because of the cost of CO2, we could help create demand for high-volume CO2?

No uses that do not involve full recovery/recycling of CO2, please..

One obvious sink for the CO2 is, of course, greenhouses. CO2 is a Greenhouse Gas after all :)

On 3 October 2018 00:01:06 GMT+01:00, Tito <titoja...@gmail.com> wrote:

Agreed, Patrik, burying fossil fuels sounds like a plan, I know a place right down the street that sells liquid fuel!

And yes, smokestack is way more energetically efficient than sucking it straight from the air, carbon dioxide at industrial smokestack "can be as much as 10−25 volume percent or more of the flue gas". The main limitation seems to be that the uses of concentrated carbon dioxide are limited.

Tito

On Friday, September 21, 2018 at 10:39:38 PM UTC-7, Patrik D'haeseleer wrote:

The biggest problem with carbon capture and sequestration (CCS) is that the efficiencies go down dramatically the more diluted the carbon is. So capturing CO2 at a smokestack is way more efficient than sucking it straight from the air. 

By extrapolation, by far the most efficient form of carbon capture and sequestration would be to capture the carbon in its purest form. That is, take coal and other fossil fuels, and bury them underground!

Patrik

--
Sent from my Android device with K-9 Mail. Please excuse my brevity.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/054c68a8-f9b4-4a4e-b12b-6568c280e0e7%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

Sorry to necrobump a clearly dead thread, but I am actually specifically in need of this literally right now. In regards to Josiah's question, I'll run validation tests (can test lysozyme and various cancer biomarkers) in exchange for the spec and design files.

Let me know ASAP, thanks.

On Wednesday, January 28, 2015 at 2:01:23 PM UTC-5, Josiah Zayner wrote:

If Daniel responds to this thread I have a question about the Kickstarter.

Have you done any experiments to validate your instrument by showing it provides measurements similar to what is seen for commercial grade instruments in literature?




On Friday, January 23, 2015 at 12:14:23 PM UTC-8, Daniel Glenn wrote:

I was wondering if their would be interest in an affordable surface plasmon resonance bio-assay sensor?

Surface plasmon resonance can be used as a method to detect antibody-antigen (and other) reactions directly with an optoelectronic sensor. Biacore and others make very expensive instruments that read 96-well plates, but I'm talking about something that reads samples one at a time and doesn't cost $33,000+.

It can be used to perform the same type of assays that occur in Western blot and ELISA without attaching tags, because the probe molecule is immobilized chemically on the surface of the optics with chemistry. Would those type of assays be useful to the general DIYbio community?

Do you think that there would be interest in such a device for the more general citizen-science community if test kits for things like Salmonella or Dengue that anyone with reasonable skills and training could use were developed?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/85a63cea-fc23-4d43-ba9d-837c43658f28%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.