Hi guys,
-- I am currently re-visiting the idea of chloroplast engineering. Chloroplast engineering yields loads of proteins, and is only maternally inherited (containment bonus). Antibiotic selection marker will be removable with Cre-Lox, and should happen with low frequency by iself too.
First project: creating the empty vector, SciHouse in Florida will store the vector in bulk and share with anyone interested (open source)
Second project: putting the lux operon in, just because. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015461 *
I have seen one of these plants, and they were visibly glowing to the naked eye (but weak). Note that that was way before the Silicon Valley glowing plant cowboy project.
*Unfortunately they stopped selling them in the United States for no apparent reason (maybe they didnt't get enough customers).
Not sure if I should start with weeping willow or tobacco. I've been routinely working with tobacco and can share my protocols with our US collaborators so they can do the tissue culture there. Tobacco is pretty lame though.
Anyways, just wanted to see if anyone would be willing to chime in, synthesis is expected to cost around 500$. Attached the file. Addition of lux will just be one PCR step
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