Hi,
So I briefly looked into this today, and found that western blot is not the recommended diagnostic tool for Lyme. Its mostly used as a confirmation if other tests (mostly an ELISA) are positive to rule out false positives. It is mainly looking at a messy blot with no standard interpretation. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3250769/
It does not seem like a good idea for me to do this as firstly the results will show some bands either way, and secondly, it has a lot of variability even between labs, so there is no standard interpretation. https://academic.oup.com/cid/article/57/3/341/460648
Since its a difficult, messy blot it is probably not a good choice for a first western blot either. There are easier, cleaner things to blot for, like tubulin or histones. Those are much better targets for a first blot if you are not familiar with the technique yet.
Also of note, health and safety rules in almost any lab will forbid researchers to perform any testing on their own samples. Reason being that if the sample gets contaminated during handling, then the contamination has successfully attacked the tissue of the researcher in the dish, and is likely able to infect the person as well. Because of this you should never be allowed to handle your own samples. In our lab we perform experiments with human samples, including sometimes each others, so there is a rota system in place to avoid any researcher meeting their own tissue in culture.
Cheers,
Mate
Anyone ever done any Lyme disease related testing on themselves? I've found PCR primers for Borrelia but IgM and IgG seem to be a more trusted test outside of an ELISA specific to Borrelia.Rather curious. I've had the bullseye rash 3 times, doctors only treated with doxycycline 1 time, and all ticks removed in under 24 hours (which "they" say means Borrelia cannot be transferred). Doctors also always refused to do a lab test as they said the rash was confirmation for needing antibiotics.There's still a lot of debate about chronic lyme and false positives with tests but, not here to voice opinion on that, mainly interested in doing my first Western.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/39c94411-551f-4ed8-8604-329271b33b23%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment