Typically, culturing animal cells requires a water jacketed incubator with CO2 supply, an HEPA filtered laminar flow hood, and an autoclave for media prep and waste disposal, and a microscope. The pipets and culture dishes used need to be sterile, so either disposable or glass and autoclaved. Typically, the culture media is expensive. Insect cells are somewhat easier to work with, but most mammalian cells require antibiotics and animal serum.
Cell culture typically is done in a dedicated space to keep mold and bacterial spores down, and the consumables are expensive. Cell cultures are typically kept frozen in liquid nitrogen. Active cultures need attention every day or two, and are moved to new plates every few days.
Cheers,
Jim Lund
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/fd2c828e-13d8-42f7-8bd7-6c9a82e32394%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment