1) "rDNA" is a very broad topic built on many methods. I believe the safety is more related to the DNA inputs (i.g. genes) than the recombinant aspect of genome engineering. The government and nucleic acid providers monitor sequences and look for potentially biohazardous purchases.
2) Can you elaborate on how synthetic nucleic acids in humans will alter the environment? I believe this is possible but I think the statement is more bigger than you imagine and it is not intrinsically dangerous/bad.
3/4) Bleach destroys DNA. Please, be more specific about "rDNA".
I believe you don't understand what "recombinant DNA" means these days. I hope you educate yourself before making more statements like this, "Matthew Endrizzi, a biology teacher in New Hampshire, suggested recombinant DNA research—including CRISPR—was dangerous enough in theory that he has proposed to move it all to the moon (he has not yet secured the funding or political will to do this). "
Try reading this: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5178364/
On Wed, May 22, 2019 at 3:36 PM Matt Endrizzi <matt.endrizzi@gmail.com> wrote:
I hope folks might comment on the security measures taken by the DIYbio community to ensure containment of recombinant DNA. My background is in molecular biology at Florida State, Harvard Med, and the Whitehead Institute (currently Broad). I have several concerns:
1) The biological community in general seems to have concluded that rDNA is not hazardous because nothing noticeably bad has happened in the last 40 years. Look at figure 2 in this paper:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898234/
In short, it has been shown through viral sequence analysis that plasmid DNA and other bacterial DNA can evolve into a eukaryotic virus. So plasmids we make today can contribute to viruses in the future. Risk increases with time as well as trials.
40 years is not long enough to conclude rDNA is safe.
2) Bioethics conversations focus on CRISPR application in humans. Should we be considering how our synthetic nucleic acids might affect the ecosystem that supports us? We are making nucleotide sequences that nature would likely never make.
3) I have taught high schoolers now for 15 years. If you want to know how an experiment can fail, have high schoolers do it. Whether through malice or inattention, students often make mistakes making solutions, much less performing ligation reactions or bacterial transformations. They are also not good at cleaning up. What is the competency level in your DIY lab?
4) What assurances can the DIYbio community give that BSL1 safety levels are being met and that rDNA and everything it touches are being sterilized properly?
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