Re: [DIYbio] Free of cost plasmid vector

the-odin.com has super-cheap plasmids, antibiotics, and media. They're
open to appeals from poor students/beginners too. If you post your
experiments on social media, they also give you store credit.

On Sun, Sep 22, 2019 at 8:10 PM Tom De Medts <tdmedts@gmail.com> wrote:
>
> Dear DIYbio community members,
>
> Greetings from the Richmond area, Virginia, USA !
>
> Where / from who can I request, free-of-cost, a small-sized plasmid (<5KBp) that I need to practice / teach the E. coli bacterial transformation protocol ?
>
> I prefer that the plasmid be selected using Kanamycin, because I got a gift of some Kanamycin powder from a retired professor.
>
> Ampicillin selection is also OK I suppose, since I think I should be able to buy some from Amazon, but I prefer not to have that expense.
>
> Thanks, in advance.
> - Tom
>
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Re: [DIYbio] Re: Modifying orientation?

Ouch yeah...
You've got to have something missing in your brain to try that on another human

Le jeu. 26 sept. 2019 à 16:50, <cathalgarvey@cathalgarvey.me> a écrit :
Historically it has never worked for sexual orientation or gender identity, but I'll assume you were kidding. :)

Bear in mind that people on the receiving end of these "therapies" often end up with varying degrees of trauma or self-loathing, so it's rarely taken well, as a joke. I'm not one of them, but this large list probably has a lot of people who have been on the receiving end of a "pavlov way" attempt to change their orientation or gender identity.


September 26, 2019 2:05 PM, "Al" <aldric.liottier@gmail.com> wrote:
Well the pavlov way can always work ^^

Le mercredi 4 septembre 2019 17:45:16 UTC+2, SAS a écrit :
https://www.google.co.uk/amp/s/amp.reddit.com/r/Biohackers/comments/aawykp/modifying_sexual_orientation/

Biohackers discusses modifying orientation. What do people think about the possibilities?
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Re: [DIYbio] Re: Modifying orientation?

Historically it has never worked for sexual orientation or gender identity, but I'll assume you were kidding. :)

Bear in mind that people on the receiving end of these "therapies" often end up with varying degrees of trauma or self-loathing, so it's rarely taken well, as a joke. I'm not one of them, but this large list probably has a lot of people who have been on the receiving end of a "pavlov way" attempt to change their orientation or gender identity.

September 26, 2019 2:05 PM, "Al" <aldric.liottier@gmail.com> wrote:
Well the pavlov way can always work ^^

Le mercredi 4 septembre 2019 17:45:16 UTC+2, SAS a écrit :
https://www.google.co.uk/amp/s/amp.reddit.com/r/Biohackers/comments/aawykp/modifying_sexual_orientation/

Biohackers discusses modifying orientation. What do people think about the possibilities?
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[DIYbio] Re: Modifying orientation?

Well the pavlov way can always work ^^

Le mercredi 4 septembre 2019 17:45:16 UTC+2, SAS a écrit :
https://www.google.co.uk/amp/s/amp.reddit.com/r/Biohackers/comments/aawykp/modifying_sexual_orientation/

Biohackers discusses modifying orientation. What do people think about the possibilities?

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[DIYbio] HDL replacement therapy

im interested in doing my own HDL replacement therapy. Does anyone here have experience with selective HDL-delipidation or simple HDL extraction and reinfusion?

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Re: [DIYbio] Re: Chemical autoflorescence in fish

The black light is clearly visible as a light blue glow all around the fish tank.
You can see the casing of the fluorescent flashlight in many of the photos.
You can also see non-fish objects fluorescing on this page.
The rocks are phosphorescent since the glow exponentially decays over the next 4 to 5 hours.
The fish are probably just fluorescent, but the implication given by the rocks is that they are as phosphorescent as the rocks.
The parts of the fish that are not fluorescing are either heavily pigmented or lacking in calcium.
All of the fish in the tank seem to have been treated, some are more pigmented than others.

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On Tue, Sep 24, 2019 at 5:00 AM Christopher Monaco <cmonaco.hr@gmail.com> wrote:
I think this is the claim that's a bit odd:

"This's not the GloFish you've seen at most pet aquarium shop in the past decade, our Fluorescent literally lite up the surrounding area like lights bulb."

Seems to indicate, and based on images from their website, that you don't need black light. They just glow "like lights bulb."

On Monday, September 23, 2019 at 2:31:07 PM UTC-4, Yuriy wrote:
Detergent molecules bonded to scales. That's interesting. 

On Monday, September 23, 2019 at 12:30:44 PM UTC-4, Simon Quellen Field wrote:
Fluorescence is far more common than most people realize.
If you obtain a 405 nanometer (violet) laser pointer, you can detect fluorescence in wines, beer, wood, chlorophyll, and many other natural objects and substances.
It makes a good party trick.

Think of all the detective shows that use black lights to detect semen and urine on bedsheets.
People bring black lights to motels to check bathrooms for effective house cleaning.

Blue fluorescent dye is used in laundry detergent to make yellowed clothing look white.
The dye adheres well to most fabrics. I would expect it would also adhere well to fish scales.
Bring your black light into the laundry room and look at all of the splashes on the walls.

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On Mon, Sep 23, 2019 at 6:15 AM Dakota Hamill <dko...@gmail.com> wrote:

On Mon, Sep 23, 2019 at 8:04 AM Christopher Monaco <cmon...@gmail.com> wrote:
I'm skpeptical

On Sunday, September 22, 2019 at 4:50:06 AM UTC-4, Yuriy wrote:
Any guess what this could be?
https://www.aquafluorescent.com/

The site claims the fish is not GMO and they are not tattooed. Somehow the dye, whatever it is bonds to calcium rich biominirals. Bone, shell, etc.

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[DIYbio] Re: Drug addiction biohacking


RE: only new neurons

On Friday, September 20, 2019 at 3:23:39 AM UTC+2, louie anderson wrote:
...on top of the fact that if you do this via genetic egineering than only the new nerve cells will exhibit the traits you are looking for

On Friday, September 20, 2019 at 3:56:06 PM UTC+2, ruphos wrote:
The "new cells" are the cells that have been altered via CRISPR to have whatever the new material you inserted. Existing neurons won't be affected. So while adult neurogenesis does happen, the maximum effect (assuming it even worked) is going to be restricted to newly grown cells.

This doesn't sound right to me. Why would your neural stem cells have been affected by your attempt at adult genetic modification, but your ordinary neurons have been passed over? Unless you're injecting pre-modified stem cells into the brain rather than modifying existing cells with a viral vector or something.

If you can modify the DNA of existing neurons, then my judgment is that you should see the changes in the phenotype even of existing neurons. You don't need to regrow the cell from scratch for the new DNA to be used; it would only take so long as the turn-over rate of your receptor proteins, which I think isn't that long. If you cannot modify existing cells, then you're not going to get anything at all into the brain without a surgey-requiring stem cell transplant, not even when new neurons mature.

RE: D-cycloserine
On Monday, September 23, 2019 at 10:27:16 AM UTC+2, Darlene Aldente wrote:
Check out D-Cycloserine. a search of "d-cycloserine and addiction" in pub med or google scholar will give you a ton of recent peer reviewed articles. This is an old tuberculosis antibiotic that has been discovered to act on the glutamate receptor int the brain which is central to the neuroplasticity disruption associated with addition. It has shown real results when combined with CBT and the CBT sessions are times with ingestion of the drug. Same method has shown great results for war related PTSD. because the drug increases neuroplasticity, and for other reasons, it has a measurable positive effect on learning (if only I had this drug in college). You Do NOT need to take the full tuberculosis dose, just half. Also, I'm not sure about the duration of treatment. 
There is one BIG problem however: the cost. This drug has recently fallen prey to opportunistic "investors."  in 2015 the price of cycloserine increased from $500 for 30 pills to $10,800 Rodelis Therapeutics purchased it. Purdue University, the previous owner, which retained "oversight of the manufacturing operation" intervened and Rodelis returned the drug to an NGO of Purdue University foundation whi8ch now charges $1,050 for 30 capsules. If you discover a way to use the NGO system through wich governments and non-profits obtain the drug for a fraction of the price please let us know!!

If the OP were interested in this and the cost were the main obstacle, I'd suggest just going with D-serine instead. It has the same neural mechanism of action and some similar experimental results in cases when both have been used in the same type of experiment, which I'm NOT claiming includes addiction. It's available as a nootropic supplement and ridiculously affordable by comparison; plus, you avoid taking needless antibiotics. I researched this question moderately thoroughly for a tDCS project at some point. 

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Re: [DIYbio] Drug addiction biohacking

RE: only new neurons

On Friday, September 20, 2019 at 3:23:39 AM UTC+2, louie anderson wrote:
...on top of the fact that if you do this via genetic egineering than only the new nerve cells will exhibit the traits you are looking for

On Friday, September 20, 2019 at 3:56:06 PM UTC+2, ruphos wrote:
The "new cells" are the cells that have been altered via CRISPR to have whatever the new material you inserted. Existing neurons won't be affected. So while adult neurogenesis does happen, the maximum effect (assuming it even worked) is going to be restricted to newly grown cells.

This doesn't sound right to me. Why would your neural stem cells have been affected by your attempt at adult genetic modification, but your ordinary neurons have been passed over? Unless you're injecting pre-modified stem cells into the brain rather than modifying existing cells with a viral vector or something.

If you can modify the DNA of existing neurons, then my judgment is that you should see the changes in the phenotype even of existing neurons. You don't need to regrow the cell from scratch for the new DNA to be used; it would only take so long as the turn-over rate of your receptor proteins, which I think isn't that long. If you cannot modify existing cells, then you're not going to get anything at all into the brain without a surgey-requiring stem cell transplant, not even new neurons.

RE: D-cycloserine
On Monday, September 23, 2019 at 10:27:16 AM UTC+2, Darlene Aldente wrote:
Check out D-Cycloserine. a search of "d-cycloserine and addiction" in pub med or google scholar will give you a ton of recent peer reviewed articles. This is an old tuberculosis antibiotic that has been discovered to act on the glutamate receptor int the brain which is central to the neuroplasticity disruption associated with addition. It has shown real results when combined with CBT and the CBT sessions are times with ingestion of the drug. Same method has shown great results for war related PTSD. because the drug increases neuroplasticity, and for other reasons, it has a measurable positive effect on learning (if only I had this drug in college). You Do NOT need to take the full tuberculosis dose, just half. Also, I'm not sure about the duration of treatment. 
There is one BIG problem however: the cost. This drug has recently fallen prey to opportunistic "investors."  in 2015 the price of cycloserine increased from $500 for 30 pills to $10,800 Rodelis Therapeutics purchased it. Purdue University, the previous owner, which retained "oversight of the manufacturing operation" intervened and Rodelis returned the drug to an NGO of Purdue University foundation whi8ch now charges $1,050 for 30 capsules. If you discover a way to use the NGO system through wich governments and non-profits obtain the drug for a fraction of the price please let us know!!

If the OP were interested in this and the cost were the main obstacle, I'd suggest just going with D-serine instead. It has the same neural mechanism of action and some similar experimental results in cases when both have been used in the same type of experiment, which I'm saying includes addiction. It's available as a nootropic supplement and ridiculously affordable by comparison; plus, you avoid taking needless antibiotics. I researched this question moderately thoroughly for a tDCS project at some point. 

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Re: [DIYbio] Re: Chemical autoflorescence in fish

Not the detergent. A dye that is similar to the dye added to detergent.
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On Mon, Sep 23, 2019 at 11:31 AM Yuriy <yuriyology@gmail.com> wrote:
Detergent molecules bonded to scales. That's interesting. 

On Monday, September 23, 2019 at 12:30:44 PM UTC-4, Simon Quellen Field wrote:
Fluorescence is far more common than most people realize.
If you obtain a 405 nanometer (violet) laser pointer, you can detect fluorescence in wines, beer, wood, chlorophyll, and many other natural objects and substances.
It makes a good party trick.

Think of all the detective shows that use black lights to detect semen and urine on bedsheets.
People bring black lights to motels to check bathrooms for effective house cleaning.

Blue fluorescent dye is used in laundry detergent to make yellowed clothing look white.
The dye adheres well to most fabrics. I would expect it would also adhere well to fish scales.
Bring your black light into the laundry room and look at all of the splashes on the walls.

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On Mon, Sep 23, 2019 at 6:15 AM Dakota Hamill <dko...@gmail.com> wrote:

On Mon, Sep 23, 2019 at 8:04 AM Christopher Monaco <cmon...@gmail.com> wrote:
I'm skpeptical

On Sunday, September 22, 2019 at 4:50:06 AM UTC-4, Yuriy wrote:
Any guess what this could be?
https://www.aquafluorescent.com/

The site claims the fish is not GMO and they are not tattooed. Somehow the dye, whatever it is bonds to calcium rich biominirals. Bone, shell, etc.

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Re: [DIYbio] Re: Chemical autoflorescence in fish

I think this is the claim that's a bit odd:

"This's not the GloFish you've seen at most pet aquarium shop in the past decade, our Fluorescent literally lite up the surrounding area like lights bulb."

Seems to indicate, and based on images from their website, that you don't need black light. They just glow "like lights bulb."

On Monday, September 23, 2019 at 2:31:07 PM UTC-4, Yuriy wrote:
Detergent molecules bonded to scales. That's interesting. 

On Monday, September 23, 2019 at 12:30:44 PM UTC-4, Simon Quellen Field wrote:
Fluorescence is far more common than most people realize.
If you obtain a 405 nanometer (violet) laser pointer, you can detect fluorescence in wines, beer, wood, chlorophyll, and many other natural objects and substances.
It makes a good party trick.

Think of all the detective shows that use black lights to detect semen and urine on bedsheets.
People bring black lights to motels to check bathrooms for effective house cleaning.

Blue fluorescent dye is used in laundry detergent to make yellowed clothing look white.
The dye adheres well to most fabrics. I would expect it would also adhere well to fish scales.
Bring your black light into the laundry room and look at all of the splashes on the walls.

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On Mon, Sep 23, 2019 at 6:15 AM Dakota Hamill <dko...@gmail.com> wrote:

On Mon, Sep 23, 2019 at 8:04 AM Christopher Monaco <cmon...@gmail.com> wrote:
I'm skpeptical

On Sunday, September 22, 2019 at 4:50:06 AM UTC-4, Yuriy wrote:
Any guess what this could be?
https://www.aquafluorescent.com/

The site claims the fish is not GMO and they are not tattooed. Somehow the dye, whatever it is bonds to calcium rich biominirals. Bone, shell, etc.

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Re: [DIYbio] Re: Drug addiction biohacking

True true. Few success stories, indeed. That's why we want to go this route. There's another way you can omit these receptors (allegedly), and that's with ultrasound frequencies. We're just beginning this research, and obviously we don't know what will work and what won't yet.

On Monday, September 23, 2019 at 9:25:04 PM UTC-4, Cathal Garvey wrote:
I'll be blunt and say that to me this looks like a dead end, as far as a practical route. The research you linked was (going by the abstract at least) only examining one system, and cannot have gathered enough data on the other systems or the broader effects to rule out harm.

Even their own abstract ends with: "We suppose that the effect of microwaves is related to the stress reactions of the organism."

This might mean a mild and localised heat stress, or microwave-induced blood-brain-barrier leakage and consequent inflammation, or an increase in ROS and radical formation or misfolding due to enzyme damage. All are possible outcomes IMO from a blanket application of energising radiation.

I would be on the side of preferring social routes to solving addiction epidemics: basic income shows promise, better regulation of drug marketing and decriminalisation/medicalisation seem to be good policies, and there is research into older/newer pharmacological interventions pike psychedelic therapy.

But if a "medicalised silver bullet" is the goal then my money would be on small molecules in this case, as the medicine needs to cross the BBB and temporary antagonism of the right systems might be the simplest way to support behavioural therapies.

But then.. we've been there for years already and have few success stories so far.

On 24 September 2019 01:51:36 GMT+01:00, jackson parks <jacksonpar...@gmail.com> wrote:
I'll show what I'm referring to:



On Monday, September 23, 2019 at 7:50:36 PM UTC-4, Cathal Garvey wrote:
I'm no biophysicist but.. I'm finding it hard to imagine a way to use microwaves to reliably target only a single protein or conjugate. Even with a mythological control over frequency and amplitude, I'm assuming there would be a very diverse set of proteins and such that would absorb some of it, not to mention the background water and other small molecules?

On 23 September 2019 20:22:36 GMT+01:00, jackson parks <jacksonpar...@gmail.com> wrote:
Well the bottom line is, we're looking to experiment with switching off the GABA receptor's capacity to allow xenobiotic agonists to bind to them, also looking to do this with things like microwaves. We need to have the capacity to shut off only the parts of things like GABA and NMDA to disallow certain chemicals to be agonists/antagonists for them. This doesn't involve warfare, there are some people who are too addicted for rehab or anything similar to do anything for them. We're looking to go into the nervous system and actually shut off parts of the CNS so that drugs can't do anything to them - and we want a way to reverse the process to reestablish everything after they have recovered. I know this is radical, and I'm not familiar with anyone who's accomplished this, but hopefully this gets some sort of a ball rolling.

On Monday, September 23, 2019 at 2:49:21 PM UTC-4, Chris Santos-Lang wrote:
I am worried that applying biology to drug addiction is like applying nuclear fission to warfare. Drug addiction and war are social problems, not biological or physical problems, so bringing in biology and physics really just escalates the arsenal.

How's about applying science to analyze and correct the social problem?

Best Wishes,

Chris Santos-Lang

On Mon, Sep 23, 2019 at 3:27 AM Darlene Aldente <darlene...@gmail.com> wrote:
Check out D-Cycloserine. a search of "d-cycloserine and addiction" in pub med or google scholar will give you a ton of recent peer reviewed articles. This is an old tuberculosis antibiotic that has been discovered to act on the glutamate receptor int the brain which is central to the neuroplasticity disruption associated with addition. It has shown real results when combined with CBT and the CBT sessions are times with ingestion of the drug. Same method has shown great results for war related PTSD. because the drug increases neuroplasticity, and for other reasons, it has a measurable positive effect on learning (if only I had this drug in college). You Do NOT need to take the full tuberculosis dose, just half. Also, I'm not sure about the duration of treatment.
There is one BIG problem however: the cost. This drug has recently fallen prey to opportunistic "investors."  in 2015 the price of cycloserine increased from $500 for 30 pills to $10,800 Rodelis Therapeutics purchased it. Purdue University, the previous owner, which retained "oversight of the manufacturing operation" intervened and Rodelis returned the drug to an NGO of Purdue University foundation whi8ch now charges $1,050 for 30 capsules. If you discover a way to use the NGO system through wich governments and non-profits obtain the drug for a fraction of the price please let us know!!



On Thursday, September 19, 2019 at 5:40:44 PM UTC-4, jackson parks wrote:
Hello,

Thank you for letting me in. I'm Jack. A friend of mine named Jonathyn recently quit the idea of engaging in CRISPR CAS9 technology because he found out that it can't do anything for adult humans. We've been trying to find a way to help people with drug addiction, and we got introduced to biohacking. We're interested in being able to change people's nervous systems either through microwave radiation (https://www.ncbi.nlm.nih.gov/pubmed/1312845), CRISPR (if it can do anything at all), or whatever other means that don't technically need surgical procedures.

Thank you all, Jack.

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Re: [DIYbio] Re: Drug addiction biohacking

I'll be blunt and say that to me this looks like a dead end, as far as a practical route. The research you linked was (going by the abstract at least) only examining one system, and cannot have gathered enough data on the other systems or the broader effects to rule out harm.

Even their own abstract ends with: "We suppose that the effect of microwaves is related to the stress reactions of the organism."

This might mean a mild and localised heat stress, or microwave-induced blood-brain-barrier leakage and consequent inflammation, or an increase in ROS and radical formation or misfolding due to enzyme damage. All are possible outcomes IMO from a blanket application of energising radiation.

I would be on the side of preferring social routes to solving addiction epidemics: basic income shows promise, better regulation of drug marketing and decriminalisation/medicalisation seem to be good policies, and there is research into older/newer pharmacological interventions pike psychedelic therapy.

But if a "medicalised silver bullet" is the goal then my money would be on small molecules in this case, as the medicine needs to cross the BBB and temporary antagonism of the right systems might be the simplest way to support behavioural therapies.

But then.. we've been there for years already and have few success stories so far.

On 24 September 2019 01:51:36 GMT+01:00, jackson parks <jacksonparks11191992@gmail.com> wrote:
I'll show what I'm referring to:

https://www.ncbi.nlm.nih.gov/pubmed/8962884


On Monday, September 23, 2019 at 7:50:36 PM UTC-4, Cathal Garvey wrote:
I'm no biophysicist but.. I'm finding it hard to imagine a way to use microwaves to reliably target only a single protein or conjugate. Even with a mythological control over frequency and amplitude, I'm assuming there would be a very diverse set of proteins and such that would absorb some of it, not to mention the background water and other small molecules?

On 23 September 2019 20:22:36 GMT+01:00, jackson parks <jacksonpar...@gmail.com> wrote:
Well the bottom line is, we're looking to experiment with switching off the GABA receptor's capacity to allow xenobiotic agonists to bind to them, also looking to do this with things like microwaves. We need to have the capacity to shut off only the parts of things like GABA and NMDA to disallow certain chemicals to be agonists/antagonists for them. This doesn't involve warfare, there are some people who are too addicted for rehab or anything similar to do anything for them. We're looking to go into the nervous system and actually shut off parts of the CNS so that drugs can't do anything to them - and we want a way to reverse the process to reestablish everything after they have recovered. I know this is radical, and I'm not familiar with anyone who's accomplished this, but hopefully this gets some sort of a ball rolling.

On Monday, September 23, 2019 at 2:49:21 PM UTC-4, Chris Santos-Lang wrote:
I am worried that applying biology to drug addiction is like applying nuclear fission to warfare. Drug addiction and war are social problems, not biological or physical problems, so bringing in biology and physics really just escalates the arsenal.

How's about applying science to analyze and correct the social problem?

Best Wishes,

Chris Santos-Lang

On Mon, Sep 23, 2019 at 3:27 AM Darlene Aldente <darlene...@gmail.com> wrote:
Check out D-Cycloserine. a search of "d-cycloserine and addiction" in pub med or google scholar will give you a ton of recent peer reviewed articles. This is an old tuberculosis antibiotic that has been discovered to act on the glutamate receptor int the brain which is central to the neuroplasticity disruption associated with addition. It has shown real results when combined with CBT and the CBT sessions are times with ingestion of the drug. Same method has shown great results for war related PTSD. because the drug increases neuroplasticity, and for other reasons, it has a measurable positive effect on learning (if only I had this drug in college). You Do NOT need to take the full tuberculosis dose, just half. Also, I'm not sure about the duration of treatment.
There is one BIG problem however: the cost. This drug has recently fallen prey to opportunistic "investors."  in 2015 the price of cycloserine increased from $500 for 30 pills to $10,800 Rodelis Therapeutics purchased it. Purdue University, the previous owner, which retained "oversight of the manufacturing operation" intervened and Rodelis returned the drug to an NGO of Purdue University foundation whi8ch now charges $1,050 for 30 capsules. If you discover a way to use the NGO system through wich governments and non-profits obtain the drug for a fraction of the price please let us know!!



On Thursday, September 19, 2019 at 5:40:44 PM UTC-4, jackson parks wrote:
Hello,

Thank you for letting me in. I'm Jack. A friend of mine named Jonathyn recently quit the idea of engaging in CRISPR CAS9 technology because he found out that it can't do anything for adult humans. We've been trying to find a way to help people with drug addiction, and we got introduced to biohacking. We're interested in being able to change people's nervous systems either through microwave radiation (https://www.ncbi.nlm.nih.gov/pubmed/1312845), CRISPR (if it can do anything at all), or whatever other means that don't technically need surgical procedures.

Thank you all, Jack.

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Re: [DIYbio] Re: Drug addiction biohacking

I'll show what I'm referring to:

https://www.ncbi.nlm.nih.gov/pubmed/8962884


On Monday, September 23, 2019 at 7:50:36 PM UTC-4, Cathal Garvey wrote:
I'm no biophysicist but.. I'm finding it hard to imagine a way to use microwaves to reliably target only a single protein or conjugate. Even with a mythological control over frequency and amplitude, I'm assuming there would be a very diverse set of proteins and such that would absorb some of it, not to mention the background water and other small molecules?

On 23 September 2019 20:22:36 GMT+01:00, jackson parks <jacksonpar...@gmail.com> wrote:
Well the bottom line is, we're looking to experiment with switching off the GABA receptor's capacity to allow xenobiotic agonists to bind to them, also looking to do this with things like microwaves. We need to have the capacity to shut off only the parts of things like GABA and NMDA to disallow certain chemicals to be agonists/antagonists for them. This doesn't involve warfare, there are some people who are too addicted for rehab or anything similar to do anything for them. We're looking to go into the nervous system and actually shut off parts of the CNS so that drugs can't do anything to them - and we want a way to reverse the process to reestablish everything after they have recovered. I know this is radical, and I'm not familiar with anyone who's accomplished this, but hopefully this gets some sort of a ball rolling.

On Monday, September 23, 2019 at 2:49:21 PM UTC-4, Chris Santos-Lang wrote:
I am worried that applying biology to drug addiction is like applying nuclear fission to warfare. Drug addiction and war are social problems, not biological or physical problems, so bringing in biology and physics really just escalates the arsenal.

How's about applying science to analyze and correct the social problem?

Best Wishes,

Chris Santos-Lang

On Mon, Sep 23, 2019 at 3:27 AM Darlene Aldente <darlene...@gmail.com> wrote:
Check out D-Cycloserine. a search of "d-cycloserine and addiction" in pub med or google scholar will give you a ton of recent peer reviewed articles. This is an old tuberculosis antibiotic that has been discovered to act on the glutamate receptor int the brain which is central to the neuroplasticity disruption associated with addition. It has shown real results when combined with CBT and the CBT sessions are times with ingestion of the drug. Same method has shown great results for war related PTSD. because the drug increases neuroplasticity, and for other reasons, it has a measurable positive effect on learning (if only I had this drug in college). You Do NOT need to take the full tuberculosis dose, just half. Also, I'm not sure about the duration of treatment.
There is one BIG problem however: the cost. This drug has recently fallen prey to opportunistic "investors."  in 2015 the price of cycloserine increased from $500 for 30 pills to $10,800 Rodelis Therapeutics purchased it. Purdue University, the previous owner, which retained "oversight of the manufacturing operation" intervened and Rodelis returned the drug to an NGO of Purdue University foundation whi8ch now charges $1,050 for 30 capsules. If you discover a way to use the NGO system through wich governments and non-profits obtain the drug for a fraction of the price please let us know!!



On Thursday, September 19, 2019 at 5:40:44 PM UTC-4, jackson parks wrote:
Hello,

Thank you for letting me in. I'm Jack. A friend of mine named Jonathyn recently quit the idea of engaging in CRISPR CAS9 technology because he found out that it can't do anything for adult humans. We've been trying to find a way to help people with drug addiction, and we got introduced to biohacking. We're interested in being able to change people's nervous systems either through microwave radiation (https://www.ncbi.nlm.nih.gov/pubmed/1312845), CRISPR (if it can do anything at all), or whatever other means that don't technically need surgical procedures.

Thank you all, Jack.

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Re: [DIYbio] Re: Drug addiction biohacking

I'm no biophysicist but.. I'm finding it hard to imagine a way to use microwaves to reliably target only a single protein or conjugate. Even with a mythological control over frequency and amplitude, I'm assuming there would be a very diverse set of proteins and such that would absorb some of it, not to mention the background water and other small molecules?

On 23 September 2019 20:22:36 GMT+01:00, jackson parks <jacksonparks11191992@gmail.com> wrote:
Well the bottom line is, we're looking to experiment with switching off the GABA receptor's capacity to allow xenobiotic agonists to bind to them, also looking to do this with things like microwaves. We need to have the capacity to shut off only the parts of things like GABA and NMDA to disallow certain chemicals to be agonists/antagonists for them. This doesn't involve warfare, there are some people who are too addicted for rehab or anything similar to do anything for them. We're looking to go into the nervous system and actually shut off parts of the CNS so that drugs can't do anything to them - and we want a way to reverse the process to reestablish everything after they have recovered. I know this is radical, and I'm not familiar with anyone who's accomplished this, but hopefully this gets some sort of a ball rolling.

On Monday, September 23, 2019 at 2:49:21 PM UTC-4, Chris Santos-Lang wrote:
I am worried that applying biology to drug addiction is like applying nuclear fission to warfare. Drug addiction and war are social problems, not biological or physical problems, so bringing in biology and physics really just escalates the arsenal.

How's about applying science to analyze and correct the social problem?

Best Wishes,

Chris Santos-Lang

On Mon, Sep 23, 2019 at 3:27 AM Darlene Aldente <darlene...@gmail.com> wrote:
Check out D-Cycloserine. a search of "d-cycloserine and addiction" in pub med or google scholar will give you a ton of recent peer reviewed articles. This is an old tuberculosis antibiotic that has been discovered to act on the glutamate receptor int the brain which is central to the neuroplasticity disruption associated with addition. It has shown real results when combined with CBT and the CBT sessions are times with ingestion of the drug. Same method has shown great results for war related PTSD. because the drug increases neuroplasticity, and for other reasons, it has a measurable positive effect on learning (if only I had this drug in college). You Do NOT need to take the full tuberculosis dose, just half. Also, I'm not sure about the duration of treatment.
There is one BIG problem however: the cost. This drug has recently fallen prey to opportunistic "investors."  in 2015 the price of cycloserine increased from $500 for 30 pills to $10,800 Rodelis Therapeutics purchased it. Purdue University, the previous owner, which retained "oversight of the manufacturing operation" intervened and Rodelis returned the drug to an NGO of Purdue University foundation whi8ch now charges $1,050 for 30 capsules. If you discover a way to use the NGO system through wich governments and non-profits obtain the drug for a fraction of the price please let us know!!



On Thursday, September 19, 2019 at 5:40:44 PM UTC-4, jackson parks wrote:
Hello,

Thank you for letting me in. I'm Jack. A friend of mine named Jonathyn recently quit the idea of engaging in CRISPR CAS9 technology because he found out that it can't do anything for adult humans. We've been trying to find a way to help people with drug addiction, and we got introduced to biohacking. We're interested in being able to change people's nervous systems either through microwave radiation (https://www.ncbi.nlm.nih.gov/pubmed/1312845), CRISPR (if it can do anything at all), or whatever other means that don't technically need surgical procedures.

Thank you all, Jack.

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