[DIYbio] Re: Cleaning methods for pipette tips

Good afternoon Mr Jordan
If you have a protocol that you follow for the below, can you please share the same with me?

On Wednesday, April 13, 2011 at 4:20:57 AM UTC+5:30, Jordan Miller wrote:
sonicating in Alconox soapy water will remove almost everything biologic that might contaminate your next sample. Then rinse. This is our preferred way to reuse most of the plastics in the lab.

also, pipette tips DO survive autoclaving, so after rinsing off the alconox with distilled water you can autoclave them in their box.

Many pipette companies will send you free samples of their tips in an autoclavable box, just ask them! If you are going to reuse tips then that one sample box should last you a good long time.

jordan

On Apr 12, 2011, at 6:41 PM, Jelmer Cnossen wrote:

> Hello DIYbio-ers!
>
> I'm still a newbie at the practical lab side of biology. I'm wondering if people know [good] methods of cleaning pipette tips for re-use.
> Obviously not for the well funded labs that just buy new tips everytime, but this would reduce costs for DIYbio labs.
> I did some google searches, but so far haven't found anything.
>
> The reason I'm asking this is because I want to work towards building a cheap DIYbio lab robot,
> and having a cleaning system would lower cost and simplify the robot (No need to switch tips)
>
> Would any of these things make sense:
> - Very small scale localised autoclaving? Normal tips would melt, but maybe metal tips could work here, or something else that is temperature-resistant.
> - Chemical cleaning of tips, but I guess that if you need chemicals you might as well use
> - Remove the biological compounds using other methods, maybe sound waves (Sonification)
>
> Please contribute any crazy ideas you might have! :)
>
> kind regards,
> Jelmer Cnossen
>
>
>
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