Re: [DIYbio] Electroporator purchase help / advice sought

On 3/5/20 11:49 AM, Tom De Medts wrote:
> Often times, I find the setup includes, if it is BioRad,
> the gene pulser,
> the capacitance extender, and
> the pulse controller,
> *but* missing the *shocking chamber*. I wonder if the cuvette chamber is the first item in the setup to breakdown? To make things
> worse, the cuvette chamber aka shock chamber costs > $500 just by itself.

I have a couple of bio-rad gene pulsers, (not the gene pulser II the first one), to compare to the culture shock electroporator
since classic electroporation recipes are done with a big cap discharging into a 30k Ohm load resistor with the cuvette in
parallel with that load, (and the loaded cuvette is usually going to be a good bit more resistive than the standard Bio_rad 30k
Ohms). These first ones have pulse extender units available on ebay for under $100. It does not come with a safety shrouded
cuvette holder, just two universally available banana jacks for the output volts and current. You can rig up anything springy on
a plastic base to do the cuvette holding.

I've been needing to do some characterization of them and send one out to Nathan, and the other could go to you...for $50+
shipping $25. After characterizing a gene pulser I, I'll be looking for a gene pulser II like you were seeing high prices for,
since more recipes use that, and culture shock will need some guides on how to get similar results to those many many old recipes
using gene pulser I & II... I think with an ebay search going I'll find a II, (with cuvette holder), for under $300 in a few months.

The culture shock electroporator is a prototype that can shock any old amount or conductivity of stuff tossed into a cuvette even
if under-filled to the correct field strength that will perfectly porate cells. It does so by measuring before it lets rip. It
also takes a time recording, (by being a digital oscilloscope), so you can fire off blasts to cuvettes willy nilly and if you
label them, figure it out later or delegate that figuring to someone else. If that sounds useful, email me for more info. It's
not ready to go in a normal lab setting yet -- no case, no pushbuttons -- but more on that before year end.

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