Re: [DIYbio] Re: Remote design and execution of diy bio experiments

On 6/3/20 1:05 AM, Jonathan Cline wrote:
Consider the main problem
> to be the reliability of sucking up a microliter of liquid and depositing a single, specifically-sized droplet into a very tiny tube,
> as well as the repeatability of this operation.

> Consider the more real problems to be precise humidity and temperature control of the lab room, keeping the camera lenses clean,
> avoiding arm collisions due to equipment or protocol error, etc.

Thanks for these reality checks. Do you think the error and repeatability of pipettors is from
the air over liquid methods, or something else? I've seen some positive-displacement-syringes that use no air, just a metal wire
plunger in a close fitting glass tube. Is that something that needs more testing for automated liquid handling success? Or is
there a need for a better automatable seal between pipettor tip and pipettor -- maybe with an O-ring?

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