Hi everyone!
I have a conceptual question for Prime-Editing and Zinkfinger Nucleases.
Let's say ZFN:
1) You take a natural protein domain that binds DNA of a specific sequence.
2) You add a Nuclease domain to the above metnione protein.
Why doesn't the Nuclease Domain always cut any DNA when it bumps into it, and just when the DNA-binding domain binds DNA? (Unless it's FokI which needs to dimerize if I recall correctly). Is there a term that I can google to find papers?
I assume there is a conformational change that activates the nuclease domain. But how do you design this?
Same with CRISPR that changes C->T (I think they termed that CRISPR base editing). You add a deaminase to CRISPR, why doesn't the deanimase just float around in the nucleus and deanimate every base it hops by?
Any insight would be greatly appreciated!
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