Hi guys,
A question came up that I found interesting.
Electroporation is a standard method in vitro and in vivo.
The DNA swims around next to the cells, and then the electrical pulses drag the DNA through the membrane. And the membranes might also have developed pores from the shock.
Why isn't the DNA from the nucleus pulled out of the cell? Its backbone is negatively charged as well. I assume that the cell as a whole has a charge (the neagtively charged membrane builds a faraday-like cage??) and thus the cell is deformed, but the charge within the cell is homogenous?
I mean, electroporation would not work if the nuclear DNA would just be dragged out of the cell so I assume that is what heppens.
Would be cool to see if anyone has deeper insight
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