Hi Nathan et al.,
Cheers!
Tom
Yes Ebay is your friend, if engaged in DIY work. I bought a BIORAD Gene Pulser and a BIO RAD spectrophotometer for great deals.
I like both the old Perkin Elmer and MJRresearch models of PCR machines / thermocyclers. I've use them both quite a bit in the past.
My question was intended to inquire about which makes and models are sturdy even when purchased used, because it is usually not possible to
have them fixed by the manufacturer, for logistical reasons or because it's not worth the money and time. Repair cost >> Purchase cost?!
Sorry that my question was not framed clearer...
Anyways, I was not aware of BioLegio suggested by Hans Wilms. I've bookmarked it on my browser.
Just curious - what do you plan to use the primers for? (you don't need to answer that, unless you want to)
Good luck with wrapping up your PhD. My own PhD marathon felt like a sprint to tie up many loose ends, but it was worth it, looking back 5 years :)
Good luck with wrapping up your PhD. My own PhD marathon felt like a sprint to tie up many loose ends, but it was worth it, looking back 5 years :)
Cheers!
Tom
On Mon, Mar 15, 2021 at 12:45 PM Nathan Burge <nattysciencetwitch@gmail.com> wrote:
@ TomEbay is a wonderful thing :) There's a lot of DIY options out there ranging from a few papers to hackers on instructables. But if you don't want to do that, ebay always seems to have a collection of used thermocyclers. Typically it seems to be the cheapest ones are 150-200$ and are usually the MJC research PTC-100 or Techne TC-3000. I just got really lucky and happened to snag a Techne one for 50$. They are used of course so no idea how long they will end up lasting. Otherwise, there is the openPCR, miniPCR, proPCR, and pocketPCR that are hundreds of dollars. The miniPCR seems really nice to me.As far as cloning goes, I haven't looked into doing any plasmid construction yet, but I've seen some related stuff at carolina.com, minipcr.com , the-odin.com, homesciencetools.com and ebay--On Sun, Mar 14, 2021 at 8:52 PM Tom De Medts <tdmedts@gmail.com> wrote:@ Nathan,Because you are looking into buying PCR primers, I am assuming you have access to the entire pipeline in terms of equipment.So what sort of PCR / thermocycler do you have? And where did you buy it?I have a limited budget and am curious to know if the low cost offerings from companies I've never heard of while in academia, are good over extended periods of use...@ wider communityBeen outside a wet lab for some time now, stuck in front of a monitor doing bioinformatics.So wondering what easy to source cloning kits are available these days?And what cloning technique(s) is most popular to make constructs of cDNA in expression vectors for stable insertion into plant species?GoldenGate or something else?
Any good online links to get familiar with them?
Thanks in advance.Best,Tom--On Sun, Mar 14, 2021 at 3:30 PM Nathan Burge <nattysciencetwitch@gmail.com> wrote:Hi there,I'm new to the DIYBio space and almost have a PhD in biochemistry. I'm looking to order primers for my personal use and places like IDT and Sigma want you to be a university or company to order oligos? Where do people end up ordering these nowadays with no affiliations?--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/6e2ddb73-1f0d-47fd-abf0-d41502fdab8dn%40googlegroups.com.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/-wD16N3kEoI/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CANpz%3DfEz8yOdGsZ6i%2ByU2xOD-LQ4GJmtdGPwORhUUDbb8dx-dg%40mail.gmail.com.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CABBvJPEJR7OR8WNg_%2BqVQih21jEXdCoJDDjOEM00p2zqfScTMQ%40mail.gmail.com.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CANpz%3DfGkafeCSSM%2BR_F4wV10pXMtRtqFGGq-nyiUT_b1N0-Mrg%40mail.gmail.com.
0 comments:
Post a Comment