Re: Re[2]: [DIYbio] Continuous (blood) Analyte Monitoring

Dakota, Frank - thanks for your replies.

A video explaining how the CGM works - https://www.youtube.com/watch?v=1FecOjMsgMw

It's by Dr. John Mastrototaro, "father of CGM", describes how a continuous glucose monitor works for people with diabetes

And this work done in Medtronics' Diabetes Division.

I hear what you say about fertility being a complex issue, and that is why I do not want to go down that route

Instead I want to 1st focus on what is technically feasible before delving into whether results are biologically possible (cross-contamination from other steroid etc)

Here is an example 2019 paper where the aptamer is discovered using what I think is a sequential and pooled SELEX, or something like that?

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893951/ It's close to midnight here, I'll read it later and carefully.

But something caught my attention in this paper - "Binding studies demonstrated the high affinity of each selected aptamer for its respective target, and low nanomolar range dissociation constants calculated were similar to those previously reported for steroid-binding aptamers selected using traditional SELEX approaches. Finally, the selected aptamers were exploited in microtiter plate assays, achieving nanomolar limits of detection, while the specificity of these aptamers was also demonstrated. "

I've got to find out at least 2 things:

1. Are the concentrations of target analytes even lower that aptamers' nanomolar limits of detection sensitiity?

2. CGM monitors [glucose] in interstitial space, are there even target analytes in this fluid, otherwise is continuous monitoring ruled out for a sensor that has to enter the lumen of any blood vessel?

This requires a lot of reading on my part, and consultation with smart folks like you.

So if you have additional ideas, keep them coming, thanks!

Cheers,

Tom

On Fri, Apr 9, 2021 at 4:06 PM Frank <fgarc...@gmail.com> wrote:

Yeah, you're not going to find something that can do continuous
monitoring of those hormones. But if the core problem is the fertility
then that's a different matter.  I'm no expert but I do know this is an
area where a great many issues and factors can  influence things. Some
of those fall outside of what we would assume interfere with fertility
and don't usually get considered by the typical fertility "expert".



------ Original Message ------
From: "Dakota Hamill" <dko...@gmail.com>
To: "diy...@googlegroups.com" <diy...@googlegroups.com>
Sent: 4/9/2021 2:53:56 PM
Subject: Re: [DIYbio] Continuous (blood) Analyte Monitoring

>Everything is either an ELISA or ECLIA test as far as I know, with the
>steroids also offering LC-MS/MS for higher sensitivity.  I've done the
>LC-MS/MS for estradiol as well as the normal ELISA and they were >110%
>variation between the two readings.  ELISA says my E2 is high, LCMS/MS
>says it's mid-range and fine.  Plenty of other compounds can interfere
>with the enzyme linked assays.   Would be pretty cool to have a
>constant monitoring of hormones but I don't see those tests ever
>miniaturizing anytime soon.
>
>I'm not even sure how the CGM works, is it all conductance based? Some
>electrical readout? Glucose is present at what, 100mg/mL? Those
>hormones are present at levels of nanograms to picograms per 10mL.
>
>On Fri, Apr 9, 2021 at 2:07 PM Tom De Medts <tdm...@gmail.com> wrote:
>>
>>  Dear all,
>>
>>  Is there anyone with experience in designing and implementing Continuous (blood) Analyte Monitoring like the now commonly used Continuous Glucose Monitor (CGM)?
>>
>>  My question for you is based out of my own experience and frustration at fertility treatments.  My spouse's hormone profile is very different from the ideal case, and it would be nice to be able to monitor her natural cyclic fluctuation in female hormones such as
>>
>>  Estradiol
>>  Follicle Stimulating Hormone
>>  Luteinizing Hormone
>>  Progesterone
>>
>>  Online searches for continuous monitoring of these blood analytes were not available. Please note I am not referring to strip tests for use with pinprick blood tests or with urine samples. If I am wrong, and there are indeed continuous monitors for any of these hormones, please let us know.
>>
>>  Else, if you have expertise / experience in any subject area(s) related to developing such CGM-like devices, could you please dumb it down for a non-technical audience and list
>>
>>  what the low hanging fruits that could be easily achieved are
>>  what the biological challenges are
>>  what the tech challenges with measurements are
>>  and anything else...
>>
>>
>>  Thanks!
>>  Tom
>>
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